Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing  

作　　者：Zhixin Liu Yaping Zhou Jinggong Guo Jiaoai Li Zixia Tian Zhinan Zhu Jiajing Wang Rui Wu Bo Zhang Yongjian Hu Yijing Sun Yan Shangguan Weiqiang Li Tao Li Yunhe Hu Chenxi Guo Jean-David Rochaix Yuchen Miao Xuwu Sun 

机构地区：[1]State Key Laboratory of Crop Stress Adaptation and Improvement,State Key Laboratory of Cotton Biology,Key Laboratory of Plant Stress Biology,School of Life Sciences,Henan University,85 Minglun Street,Kaifeng 475001,China [2]College of Life Sciences,Shanghai Normal University,Guilin Road 100,Shanghai,200234,China [3]Departments of Molecular Biology and Plant Biology,University of Geneva,Geneva,1211,Switzerland

出　　处：《Molecular Plant》2020年第8期1178-1193,共16页分子植物（英文版）

基　　金：This research was supported by the National Natural Science Foundation of China(31670233);the Key Scientific and Technological Projects in Henan Province,China(192102110113).

摘　　要：The regulation of stomatal lineage cell development has been extensively investigated.However,a comprehensive characterization of this biological process based on single-cell transcriptome analysis has not yet been reported.In this study,we performed RNA sequencing on 12844 individual cells from the cotyledons of 5-day-old Arabidopsis seedlings.We identified 11 cell clusters corresponding mostly to cells at specific stomatal developmental stages using a series of marker genes.Comparative analysis of genes with the highest variable expression among these cell clusters revealed transcriptional networks that regulate development from meristemoid mother cells to guard mother cells.Examination of the developmental dynamics of marker genes via pseudo-time analysis revealed potential interactions between these genes.Collectively,our study opens the door for understanding how the identified novel marker genes participate in the regulation of stomatal lineage cell development.

关 键 词：molecular profiling STOMATAL development SINGLE-CELL RNA-SEQ 

分 类 号：Q94[生物学—植物学]