一株太空培育螺旋藻诱变株的抗氧化能力研究  

Study on the antioxidant capacity of Spirulina Platensis mutant induced by space-flight breeding

作　　者：杨登亮 谢佩佩 向文洲[4] 林盛杰 李传茂 YANG Deng-liang;XIE Pei-pei;XIANG Wen-zhou;LIN Sheng-jie;LI Chuan-mao(Gongdong Danz Group Co.,Ltd.,Guangzhou,Guangdong 510410,China;Guangzhou Keneng Cosmetics Scientific Research Co.,Ltd.,Guangzhou,Guangdong 510800,China;Guangzhou Baiyun Lianjia Fine Chemistry Factory,Guangzhou,Guangdong 510540,China;Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou,Guangdong 510301,China)

机构地区：[1]广东省丹姿集团有限公司,广东广州510410 [2]广州市科能化妆品科研有限公司,广东广州510800 [3]广州市白云联佳精细化工厂,广东广州510540 [4]中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室,广东广州510301

出　　处：《日用化学品科学》2020年第10期21-24,共4页Detergent & Cosmetics

基　　金：广东省重点领域研发计划(2020B1111030004);广州市科技计划项目(201807010053,201803010118,201907010018)。

摘　　要：利用太空育种方法,培育得到新型的钝顶螺旋藻诱变株H11,乙醇提取多酚活性成分,用DPPH和ABTS法检测其抗氧化和自由基清除能力。实验结果表明,野生株和诱变株螺旋藻的总多酚得率分别为59.774μg/g(干重)和87.881μg/g(干重),野生株和诱变株螺旋藻提取物的DPPH自由基清除率IC50分别为8.931和2.076μg/mL,ABTS清除率IC50分别为1.292和0.355μg/mL;阳性对照物抗坏血酸、D-α-生育酚和Trolox的DPPH清除率IC50分别为4.084,10.309和6.452μg/mL,ABTS清除率IC50分别为0.204,4.44和1.089μg/mL。诱变株表现出优于野生株螺旋藻的DPPH和ABTS清除能力,且显著优于或接近抗坏血酸、D-α-生育酚和Trolox。With the method of space-flight breeding,a new strain of Spirulina Platensis mutant H11 was cultivated.Active ingredients polyphenols were extracted from Spirulina Platensis with ethanol.The DPPH and ABTS assays were used to detect the antioxidative and free radical-scavenging capacity of polyphenols.The results showed that the total polyphenol contents of wild type and mutant Spirulina Platensis were 59.774μg/g(dry weight)and 87.881μg/g(dry weight),respectively.The DPPH scavenging rates(IC50)of wild type and mutant Spirulina Platensis were 8.931 and 2.076μg/mL,and the ABTS scavenging rates(IC50)of wild type and mutant Spirulina Platensis were 1.292 and 0.355μg/mL,respectively;the DPPH scavenging rates(IC50)of ascorbic acid,D-α-tocopherol and Trolox were 4.084,10.309 and 6.452μg/mL,respectively,and the ABTS scavenging rates(IC50)were correspondingly 0.204,4.44 and 1.089μg/mL.The mutant strain showed stronger DPPH and ABTS scavenging capacity than wild type Spirulina Platensis,and the antioxidant effect of mutant Spirulina Platensis was stronger than or close to that of ascorbic acid,D-α-tocopherol and Trolox.

关 键 词：螺旋藻 太空育种 多酚 抗氧化 DPPH自由基 ABTS自由基 

分 类 号：TQ658[化学工程—精细化工]