高温需要蛋白A对乳牙变异链球菌果糖基转移酶表达的影响  

Effect of HtrA on the fructosyltransferase expression in cariogenic Streptococcus mutans

作　　者：毛玲 杨维虹 徐仕珍 王玲 韩林秀 刘兴容 MAO Ling;YANG Weihong;XU Shizhen;WANG Ling;HAN Linxiu;LIU Xingrong(Oral&Maxillofacial Reconstruction and Regeneration Laboratory,the Affiliated Stomatology Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China;Department of Pediatric Dentistry,the Affiliated Stomatology Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China;Department of Stomatology,ManYang 404 Hospital)

机构地区：[1]西南医科大学口颌面修复重建与再生实验室,四川泸州646000 [2]西南医科大学附属口腔医院儿童口腔科,四川泸州646000 [3]四川绵阳四0四医院口腔科

出　　处：《西南医科大学学报》2020年第5期446-449,共4页Journal of Southwest Medical University

基　　金：四川省科技厅-泸州市人民政府-泸州医学院2014年联合科研项目(LY53);四川省卫生厅科研基金(100247);四川省教育厅科研基金(13ZA0235)。

摘　　要：目的:研究乳牙高致龋性变异链球菌(streptococcus mutans,S.mutans)高温需要蛋白A(high temperature requirement serine proteinase A,HtrA)基因缺陷株和HtrA高毒力株在体外非应激环境中的果糖基转移酶(fructosyltransferase,FTF)表达能力的差异。方法:选用课题前期已获得的HtrA高毒力株及以此构建的HtrA基因缺陷株、S.mutans国际标准株UA159在脑心浸出液肉汤(brain heart infusion broth,BHI)培养基培养16 h后,用实时荧光定量核酸扩增检测系统(real-time quantitative PCR detecting system,RT-qPCR),检测三菌株FTF基因的扩增情况。用相对定量分析-2-△△Ct法计算并比较HtrA基因缺陷株与HtrA高毒力株和UA159标准株FTF基因的相对表达能力的差异。结果:HtrA高毒力株的FTF基因的表达量约是HtrA基因缺陷株23.588倍;UA159标准株的FTF基因的表达量约是HtrA基因缺陷株10.556倍。结论:乳牙变异链球菌的FTF的表达受到HtrA的调控。Objective:To investigate the difference in the expression ability of fructosyltransferase(FTF)in vitro in a non-stressful environment between a high temperature requirement serine proteinase A(HtrA)gene-deficient Streptococcus mutans strain and a HtrA high-virulent S.mutans strain with high cariogenicity.Methods:A high-virulent S.mutans strain obtained in our previous study,an HtrA gene-deficient strain constructed based on it,and an international standard strain,S.mutans UA159,were selected and cultured in brain-heart infusion(BHI)broth for 16 h.The real-time quantitative PCR detection system was used to determine the amplification of FTF gene in the three strains.The relative quantitative analysis—2-△△Ct method was used to calculate and compare the relative expression of FTF gene in the three strains.Results:The FTF gene expression in the HtrA high-virulent strain was 23.588 times that in the HtrA gene-deficient strain,while that in the UA159 standard strain was 10.556 times that in the HtrA gene-deficient strain.Conclusion:The expression of FTF in cariogenic S.mutans is regulated by HtrA.

关 键 词：乳牙 龋病 变异链球菌 高温需要蛋白A 果糖基转移酶 

分 类 号：R781.1[医药卫生—口腔医学]