产碱性纤维素酶菌株WS-135的筛选及酶学性质研究  

Screening of Alkaline Cellulase Producing Strain WS-135 and Study on Its Enzymatic Characterization

作　　者：钱娟娟 王克芬 刘胜利 王帅 王兴吉 刘文龙 QIAN Juan-juan;WANG Ke-fen;LIU Sheng-li;WANG Shuai;WANG Xing-ji;LIU Wen-long(Shandong Longkete Enzyme Co.,Ltd.,Linyi Shandong 276400,China;Shandong Province Key Laboratory of Enzyme Preparation Fermentation Technology,Linyi Shandong 276400,China)

机构地区：[1]山东隆科特酶制剂有限公司,山东省酶制剂生物发酵技术重点实验室,山东临沂276400

出　　处：《食品与发酵科技》2020年第5期60-63,71,共5页Food and Fermentation Science & Technology

基　　金：山东省技术创新项目(201830515049)。

摘　　要：对实验室保藏菌种短小芽孢杆菌WS-135进行紫外诱变,筛选出产酶高的菌株A-5,酶活力为22.1U·mL^-1,较未诱变菌株提高2.37倍。以紫外诱变筛选菌株为出发菌株,进行硫酸二乙酯诱变,筛选得到一株酶活为28.9U·mL^-1的菌株B-7,较未诱变菌株提高1.3倍。B-7菌株连续传代8次,酶活力保持稳定。研究其酶学特性,结果表明温度为35℃时酶的活性最好,在pH为7-10时,有较好的酶活性;在40℃,pH 9.0、10.0条件下酶活稳定,在常温下保藏8个月后,仍有80%的酶活力,表明该酶稳定性较好。The original strain Bacillus pumilus WS-135 was mutated by UV in the paper,screening of high enzyme producing strain A-5,and its enzyme productivity was 22.1 U·mL-1 and 2.37 fold higher than that of parent strain.The mutant A-5 was mutated by DES,and a strain B-7 with 28.9 U·mL^-1 enzyme activity was obtained which was 1.3 fold higher than that of parent strain.The activity of alkaline cellulase remained stable after 8 passages.The optimal temperature and pH of alkaline cellulase were at 35℃,pH 7.0-10.0.The activity of alkaline cellulase was stable at 40℃,pH 9.0 and pH 10.0.Store for 8 months at 25℃,and enzyme activity of alkaline cellulase reduced 20%when compared with the initial enzyme activity.

关 键 词：碱性纤维素酶 诱变 酶学性质 稳定性 

分 类 号：TS201.3[轻工技术与工程—食品科学]