基于GB 4789.10-2016的银白色葡萄球菌鉴定方法的改进  

Improvement on the Identification Method of Staphylococcus argenteus Based on GB 4789.10-2016

作　　者：金方宁 张道锋[1] 区楚君 史贤明[1] 施春雷[1] Jin Fangning;Zhang Daofeng;Ou Chujun;Shi Xianming;Shi Chunlei(MOST-USDA Joint Research Center for Food Safety,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240)

机构地区：[1]上海交通大学,农业与生物学院中美食品安全联合研究中心,上海200240

出　　处：《中国食品学报》2020年第10期240-246,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十三五”国家重点研发计划项目(2017YFC1600100);国家自然科学基金项目(31671943)。

摘　　要：探讨基于食品安全国家标准GB 4789.10-2016鉴定银白色葡萄球菌(Staphylococcus argenteus)的适用性,并提出相应的改进方法。采用GB 4789.10-2016第一法与PCR鉴定方法(目的基因为nuc1或NRPS)同时对193株金黄色葡萄球菌分离株和6株银白色葡萄球菌分离株进行鉴定,比较两者结果。结果表明,GB 4789.10-2016和nuc1-PCR方法将银白色葡萄球菌错误鉴定为金黄色葡萄球菌,而NRPS-PCR方法可以区分两者。研究表明:在GB 4789.10-2016的基础上,结合NRPS-PCR建立一套检验流程,可鉴定并区分金黄色葡萄菌和银白色葡萄菌。It is aimed to investigate the applicability for identifying Staphylococcus argenteus by GB 4789.10-2016 method,and then to establish a complementary method.Total 193 isolates of Staphylococcus aureus and 6 isolates of Staphylococcus argenteus were used in the identification with the both methods either recommended in GB 4789.10-2016 or PCR amplification of nuc1 and NRPS genes.And according to the results of biochemical characteristics and PCR amplification,it was unfortunate that S.argenteus strains were all falsely detected as S.aureus with the method recommended in GB 4789.10-2016 and the nuc1-PCR method,however,the NRPS-PCR method established in this study can distinguish these two species.Therefore,a new protocol was established based on the combination of GB 4789.10-2016 and NRPS-PCR to identify and distinguish S.aureus and S.argenteus.

关 键 词：银白色葡萄球菌 金黄色葡萄球菌 GB 4789.10-2016 耐热核酸酶基因nuc1 非核糖体多肽合成酶基因NRPS 

分 类 号：TS201.3[轻工技术与工程—食品科学]