食用农产品中单核细胞增生李斯特菌的污染状况与血清组分型  

Prevalence and Serogroup of Listeria monocytogenes in Agri-food

作　　者：索玉娟[1,2,3] 林婷 瞿洋 白亚龙 沈源源 邵毅[1,3] 周昌艳 Suo Yujuan;Lin Ting;Qu Yang;Bai Yalong;Shen Yuanyuan;Shao Yi;Zhou Changyan(The Institute of Agri-food Standard and Testing Technology,Shanghai Academy of Agricultural Sciences,Shanghai 201403;Security Risk Assessment Laboratory of Ministry of Agriculture(Shanghai),Shanghai 201403;Shanghai Engineering Research Center for Quality and Safety of Agro-food,Shanghai 201403)

机构地区：[1]上海农业科学院农产品质量标准与检测技术研究所,上海201403 [2]农业农村部农产品质量安全风险评估实验室(上海),上海201403 [3]上海农产品质量安全工程技术研究中心,上海201403

出　　处：《中国食品学报》2020年第10期309-315,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金青年科学基金项目(31701714);国家农产品质量安全风险评估计划项目(GJFP2019005)。

摘　　要：单核细胞增生李斯特菌是致死率较高的一类重要食源性致病菌。本研究共采集上海市部分超市、农贸市场及生产基地的4大类(生鲜肉、鸡蛋、牛奶和果蔬)共437份食用农产品样品,按照GB 4789.30-2010方法对其中的单核细胞增生李斯特菌进行分离、鉴定,利用多重PCR方法对收集的分离株进行血清组分型。结果显示,单核细胞增生李斯特菌的总检出率为14.9%(65/437),生鲜肉、鸡蛋、牛奶和果蔬中的污染率依次为27.2%(61/224),3.4%(2/58),2.0%(1/51)和1.0%(1/104);79株单核细胞增生李斯特菌分离株可被分为5个血清组:1/2a或3a(55.7%,n=44),1/2c或3c(21.5%,n=17),1/2b或3b或7(17.7%,n=14),4a或4c(3.8%,n=3)和4b或4d或4e(1.3%,n=1)。上述结果表明:上海地区农产品中存在不同程度的单核细胞增生李斯特菌污染,且部分分离株有较高的致病性,该结果为上海地区单核细胞增生李斯特菌流行病学的风险监测提供了数据支持。Listeria monocytogenes is an important foodborne pathogen with high mortality rates.A total of 437 samples with 4 kinds of agri-food(fresh meat,egg,milk and vegetable)were collected from supermarkets,farmers markets and production base in Shanghai.L.monocytogens were isolated from these samples by GB 4789.30-2010 standard method,and the isolates serogroup was identified by multiple PCR.Results showed that total detection rate of L.monocytogenes was 14.9%(65/437).The percentage distribution of the isolates was 27.2%(61/224),3.4%(2/58),2.0%(1/51)and 1.0%(1/104)for raw meat,egg,milk and vegetable,respectively.All of 79 isolates were serotyped into five serogroups:1/2a or 3a(55.7%,n=44),1/2c or 3c(21.5%,n=17),1/2b or 3b or 7(17.7%,n=14),4a or 4c(3.8%,n=3)and 4b or 4d or 4c(1.3%,n=1).This research represents the different prevalence of L.monocytogens in raw-foods in Shanghai,and partial isolates belong to high-pathogenicity.Our results will provide the fundamental data for epidemiological monitoring of L.monocytogenes in Shanghai.

关 键 词：单核细胞增生李斯特菌 食用农产品 污染状况 血清组 风险监测 

分 类 号：TS201.3[轻工技术与工程—食品科学]