不同粒径的免疫磁珠对食源性致病菌捕获效率的影响  

Effect of the Size of Immunomagnetic Beads on Capture Efficiency for Foodborne Pathogenic Bacteria

作　　者：李静雯[1] 杜美红[1] 杨寅[1] 陈婷[1] 陈尔凝[1] 赵瑞雪[1] LI Jingwen;DU Meihong;YANG Yin;CHEN Ting;CHEN Erning;ZHAO Ruixue(Beijing Engineering Research Center of Food Safety Analysis/Beijing Engineering Technology Research Center of Gene Sequencing and Gene Function Analysis,Beijing Center for Physical&Chemical Analysis,Beijing 100089,China)

机构地区：[1]北京市理化分析测试中心北京市食品安全分析测试工程技术研究中心/北京市基因测序与功能分析工程技术研究中心,北京100089

出　　处：《食品与生物技术学报》2020年第9期46-52,共7页Journal of Food Science and Biotechnology

基　　金：北京市科学技术研究院创新团队计划项目(IG201807C1);国家重点研发计划资助项目(2016YFF0203802)。

摘　　要：粒径是影响免疫磁珠捕获效率的关键因素。选取微米级(1μm)和亚微米级(300 nm)超顺磁珠,偶联沙门氏菌多克隆抗体制备免疫磁珠。通过流式细胞仪、分光光度计分别测定抗体偶联量和磁回收率。结果表明,与微米级免疫磁珠相比,亚微米级免疫磁珠抗体偶联量较高,在缓冲液中磁回收率较高,因此捕获率较高(300 nm:55%,1μm:41%);在牛奶中磁回收率较低,导致捕获率明显降低(300 nm:15%,1μm:26%)。因此,需根据待测样本的体积与黏度来确定免疫磁珠的粒径。Particle size is a critical factor for capture efficiency(CE)of immunomagnetic beads(IMBs).IMBs were prepared by conjugating micrometer size(1μm)or submicrometer size(300 nm)of superparamagnetic beads with anti-Salmonella polyclonal antibody.The amount of coupled antibody and the recovery rate were measured by flow cytometer and spectrophotometer,respectively.The results showed that compared with micron-IMBs,submicron-IMBs had a higher amount of coupled antibody and a higher recovery rate in buffer,and the CE was higher(300 nm:55%,1μm:41%).However,submicron-IMBs had a lower recovery rate in milk,which led to asignificant reduction of the CE of submicron-IMBs(300 nm:15%,1μm:26%).Therefore,the particle size of IMBs should be determined according to the volume and viscosity of samples.

关 键 词：微米级免疫磁珠 亚微米级免疫磁珠 粒径 食源性致病菌 捕获效率 磁回收率 

分 类 号：TS207.4[轻工技术与工程—食品科学]