Enriched environment boosts the post-stroke recovery of neurological function by promoting autophagy  

作　　者：Yi-Hao Deng Ling-Ling Dong Yong-Jie Zhang Xiao-Ming Zhao Hong-Yun He 

机构地区：[1]Department of Basic Medicine,Medical School,Kunming University of Science and Technology,Kunming,Yunnan Province,China

出　　处：《Neural Regeneration Research》2021年第5期813-819,共7页中国神经再生研究（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China,Nos.81660383(to YHD),81860411(to HYH),81960418(to YHD);Yunnan Ten Thousand Talents Plan Young&Elite Talents Project of China,No.YNWR-QNBJ-2018-034(to YHD);Applied Basic Research Foundation of Yunnan Province of China,Nos.2017FB113(to YHD),2019FB098(to HYH);Science Research Fund of Yunnan Provincial Department of Education of China,No.2018JS016(to HYH).

摘　　要：Autophagy is crucial for maintaining cellular homeostasis,and can be activated after ischemic stroke.It also participates in nerve injury and repair.The purpose of this study was to investigate whether an enriched environment has neuroprotective effects through affecting autophagy.A Sprague-Dawley rat model of transient ischemic stroke was prepared by occlusion of the middle cerebral artery followed by reperfusion.One week after surgery,these rats were raised in either a standard environment or an enriched environment for 4 successive weeks.The enriched environment increased Beclin-1 expression and the LC3-II/LC3-I ratio in the autophagy/lysosomal pathway in the penumbra of middle cerebral artery-occluded rats.Enriched environment-induced elevations in autophagic activity were mainly observed in neurons.Enriched environment treatment also promoted the fusion of autophagosomes with lysosomes,enhanced the lysosomal activities of lysosomal-associated membrane protein 1,cathepsin B,and cathepsin D,and reduced the expression of ubiquitin and p62.After 4 weeks of enriched environment treatment,neurological deficits and neuronal death caused by middle cerebral artery occlusion/reperfusion were significantly alleviated,and infarct volume was significantly reduced.These findings suggest that neuronal autophagy is likely the neuroprotective mechanism by which an enriched environment promotes recovery from ischemic stroke.This study was approved by the Animal Ethics Committee of the Kunming University of Science and Technology,China(approval No.5301002013855)on March 1,2019.

关 键 词：AUTOPHAGY brain central nervous system factor INJURY PATHWAYS protection regeneration repair STROKE 

分 类 号：R459.9[医药卫生—治疗学] R741[医药卫生—临床医学]