纳米氧化锌对美国红鱼肝细胞的毒性效应及机制  

Toxic Effect and Mechanism of Nano-ZnO in Hepatocytes of Sciaenops ocellatus

作　　者：朱跃骅 张剑 钱云霞[1] Zhu Yuehua;Zhang Jian;Qian Yunxia(College of Marine Sciences,Ningbo University,Ningbo 315823,China)

机构地区：[1]宁波大学海洋学院,宁波315823

出　　处：《生态毒理学报》2020年第4期224-232,共9页Asian Journal of Ecotoxicology

基　　金：国家重点研发计划重点专项(2016YFC1402405)。

摘　　要：纳米氧化锌(ZnO NPs)的广泛应用所引发的环境与健康风险备受关注。为探究ZnO NPs对水生生物的毒性影响,以美国红鱼的原代肝细胞为实验对象,通过MTT法和中性红摄取(NRU)法检测细胞存活率,以评估ZnO NPs的细胞毒性;美国红鱼肝细胞经ZnO NPs处理6 h后,检测肝细胞内丙二醛(MDA)含量,并用流式细胞仪检测细胞对ZnO NPs的吞噬、胞内活性氧(ROS)的释放以及细胞凋亡率的变化。结果表明,美国红鱼肝细胞暴露于37.5μg·mL^-1和50μg·mL^-1 ZnO NPs 6 h后,细胞存活率较对照组显著降低,且ZnO NPs的毒性具有浓度和时间依赖性。研究表明,ZnO NPs进入细胞后,细胞内ROS产量增加,引起氧化应激,诱导细胞凋亡。The toxicity of zinc oxide nano particles(ZnO NPs)has received increasing attention in recent years.This experiment was aimed to assess the toxicity effects of ZnO NPs on primary cultured hepatocytes of Sciaenops ocellatus.The in vitro cytotoxicity of ZnO NPs was tested using MTT-fomazan assay and neutral red uptake(NRU)assay.Cellular uptake of ZnO NPs,intracellular reactive oxygen production and apoptosis rate were measured by flow cytometry after exposure to ZnO NPs for 6 h,and the content of malondialdehyde(MDA)were also detected by colorimetry.The results showed that the cell viability decreased after Sciaenops ocellatus hepatocytes were exposed to 37.5μg·mL^-1 and 50μg·mL^-1 ZnO NPs for 6 h.The studies indicated that ZnO NPs inhibited the growth of the hepatocytes of Sciaenops ocellatus in a concentration-and time-dependent manner.It is demon-strated that ZnO NPs induced cells apoptosis,which was mediated by oxidative stress as evidenced by an increase in intracellular ROS and the content of MDA generation.

关 键 词：纳米氧化锌 美国红鱼 细胞毒性 氧化应激 流式细胞 

分 类 号：X171.5[环境科学与工程—环境科学]