香椿中萜类化学成分研究  

Terpenoids from Toona sinensis

作　　者：胡疆 李佳勋 李强 彭天凤 施晓东 吕涛 蔡建 景年华 杨卫星 王巍 HU Jiang;LI Jia-xun;LI Qiang;PENG Tian-feng;SHI Xiao-dong;LYU Tao;CAI Jian;JING Nian-hua;YANG Wei-xing;WANG Wei(Institute of Characteristic Medicinal Resource of Ethnic Minorities,Qujing Normal University,Qujing 655011,China;Tumor Hospital of Yunnan Province,the Third Affiliated Hospital of Kunming Medical University,Kunming 650118,China;Department of Pharmacy,Navy Medical Center of PLA,Navy Military Medical University,Shanghai 200052,China)

机构地区：[1]曲靖师范学院少数民族特色药用资源研究所,云南曲靖655011 [2]云南省肿瘤医院,昆明医科大学第三附属医院,云南昆明650118 [3]海军军医大学海军特色医学中心药剂科,上海200052

出　　处：《中国中药杂志》2020年第18期4411-4415,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31760341);2016年和2017年云南省大学生创新创业训练计划项目。

摘　　要：采用硅胶、葡聚糖凝胶Sephadex LH-20、小孔树脂凝胶柱MCI及反相色谱柱等多种色谱技术对香椿Toona sinensis进行化学成分研究,利用1H-NMR,13C-NMR和MS等波谱学方法并结合文献对分离得到的化合物进行结构鉴定。从该植物叶子的80%乙醇提取液的乙酸乙酯萃取部分中分离得到8个萜类成分,分别鉴定为1个海松二烯型(pimaradiene)二萜:8β-hydroxypimar-15-en-19-oic acid methyl ester(1)和7个三萜类成分:cedrodorol B(2),11β-acetoxyobacunol(3),toonayunnanin D(4),toonaciliatone D(5),toonaciliatone A(6),cedrelone(7)和11β-hydroxygedunin(8)。其中,化合物1为新化合物,化合物2~7为首次从该植物中分离得到。采用MTT(噻唑蓝)比色法和DPPH(1,1-二苯基-2-三硝基苯肼)自由基清除实验对化合物1进行体外抗肿瘤和抗氧化活性测试。实验结果表明,化合物1对人类肿瘤细胞系SMMC-7721,A549和MCF-7具有一定细胞毒活性(IC50小于40μmol·L-1)和DPPH自由基清除活性(IC50为74.3μmol·L-1)。Eight terpenoids(1-8)were isolated from the ethyl acetate soluble fraction of 80%ethanol extract of leaf of Toona sinensis through various column chromatographies on silica gel,Sephadex LH-20,MCI and ODS.Their structures were elucidated as 8β-hydroxypimar-15-en-19-oic acid methyl ester(1),cedrodorol B(2),11β-acetoxyobacunol(3),toonayunnanin D(4),toonaciliatone D(5),toonaciliatone A(6),cedrelone(7),and 11β-hydroxygedunin(8)based on their chemical and physicochemical methods and spectroscopic data.Compound 1 was a new pimaradienediterpenoid and terpenoid 2-7 were isolated and identified from this plant for the first time.Compound 1 was tested in vitro for cytotoxic potential by employing MTT method and radical scavenging potential using DPPH test.As a result,1 exhibited weak cytotoxic activity against three tested tumor cell lines(SMMC-7721,A549 and MCF-7)with IC50 values less than 40μmol·L-1 and moderate radical scavenging activities with IC50 values of 74.3μmol·L-1.

关 键 词：香椿 楝科 萜类成分 抗肿瘤活性 抗氧化活性 

分 类 号：R284[医药卫生—中药学]