基于DMSA自组装单分子层的SPR免疫传感器检测沙丁胺醇  

Detection of Salbutamol by SPR Immunosensor Based on DMSA Self-assembled Monolayers

作　　者：李文杰 王晨晨 颜朦朦 李慧冬[2,3] 丁蕊艳 方丽萍[2,3] 王兰芝 侯运华[1] 杨亲正 陈子雷[2,3,4] LI Wen-jie;WANG Chen-chen;YAN Meng-meng;LI Hui-dong;DING Rui-yan;FANG Li-ping;WANG Lan-zhi;HOU Yun-hua;YANG Qin-zheng;CHEN Zi-lei(State Key Laboratory of Biobased Material and Green Papermaking,Department of Bioengineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250353,China;Institution of Quality Standard and Testing Technology for Agro-Product,Shandong Academy of Agricultural Science,Jinan 250100,China;Shandong Provincial Key Laboratory of Testing Technology for Food Quality and Security,Jinan 250100,China;College of Life Science,Shandong Normal University,Jinan 250014,China)

机构地区：[1]齐鲁工业大学(山东省科学院)生物工程学院,生物基材料与绿色造纸国家重点实验室,山东济南250353 [2]山东省农业科学院农产品质量标准与检测技术研究所,山东济南250100 [3]山东省食品质量安全检测技术重点实验室,山东济南250100 [4]山东师范大学生命科学学院,山东济南250014

出　　处：《食品工业科技》2020年第22期247-252,共6页Science and Technology of Food Industry

基　　金：山东省农业科学院农业科技创新计划(CXGC2017A03)。

摘　　要：本文通过双巯基丁二酸(DMSA)作为单分子层连接表面等离子体共振(SPR)免疫传感器中芯片上的抗原,并以沙丁胺醇(SAL)为检测模型,构建了DMSA-SPR免疫传感器,将其检测效果与传统的巯基丙酸(MPA)-SPR传感器进行比较。结果表明,室温下,DMSA-SPR免疫传感器的抗原抗体识别效率增强近一倍,其线性范围达到5~150 ng/mL,定量限(LOQ)达到5 ng/mL。此传感器应用于猪肉中SAL检测的回收率达到94.9%~108.0%,并与超高效液相色谱-串联质谱仪(UPLC-MS/MS)检测结果具有很好的相关性。因此,该方法为提高SPR免疫传感器的灵敏度提供了新思路、新方法,并可广泛应用于其他小分子的检测。In this paper,dimercaptosuccinic acid(DMSA)was used as self-assembled monolayer to connect the antigen on the chip of surface plasmon resonance(SPR)immunosensor to construct DMSA-SPR immunosensor for detection of the model of salbutamol(SAL)and its detection effect was compared with that of traditional mercaptopropionic acid(MPA)-SPR sensor.The results showed that the recognition efficiency of antigen and antibody of DMSA-SPR immunosensor was nearly doubled at room temperature,the linear range was up to 5~150 ng/mL,and the limit of quantification(LOQ)was 5 ng/mL.The recovery rate of this sensor applied to SAL detection in pork reached 94.9%~108.0%,and had a good correlation with the analysis results of ultra-high phase liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)analysis.Therefore,this method would provide a new idea and method for improving the sensitivity of SPR immunosensor,and could be widely used in the detection of other small molecules.

关 键 词：表面等离子体共振 硫醇 自组装单分子层 沙丁胺醇 

分 类 号：TS207.5[轻工技术与工程—食品科学]