基于主成分分析微波提取圆头蒿多糖工艺中单糖组分变化  

Changes of Monosaccharide Components in Microwave Extraction of Artemisia sphaerocephala Polysaccharides Based on Principal Component Analysis

作　　者：郭淑娟 朱晟永 贾悦 李敏 王露露 李丽[1] 王俊龙[1,2] 赵潇 GUO Shu-juan;ZHU Sheng-yong;JIA Yue;LI Min;WANG Lu-lu;LI Li;WANG Jun-long;ZHAO Xiao(College of Life Science,Northwest Normal University,Lanzhou 730070,China;Bioactive Products Engineering Research Center for Gansu Distinctive Plants,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070 [2]甘肃特色植物有效成分制品工程技术研究中心,甘肃兰州730070

出　　处：《食品工业科技》2020年第22期267-274,共8页Science and Technology of Food Industry

基　　金：甘肃省引导科技创新发展专项资金项目2019ZX-05。

摘　　要：为探索微波提取是否影响产物的结构特性,本研究采用主成分分析(Principal component analysis,PCA)研究方法,从统计学的角度对不同微波提取条件下得到的圆头蒿多糖(Artemisia sphaerocephala polysaccharide,ASP)进行了单糖组成分析。ASP由L-阿拉伯糖、D-木糖、D-来苏糖、D-甘露糖、D-葡萄糖、D-半乳糖六种单糖组成(摩尔比为1∶4.98∶1.69∶27.86∶3.76∶13.92),其中甘露糖和半乳糖为主要单糖组成成分。分析结果表明,微波辅助提取过程中工艺条件的改变明显影响ASP单糖组分含量的变化,其中微波提取时间对ASP主要单糖组分含量的影响最为显著,最适微波辅助提取工艺条件为辐照时间30~60 min、功率400~600 W、提取温度50~70℃。本研究为微波提取在生物质资源利用过程中提供了数据支撑和依据。In the present study,the effect of microwave irradiation on the extraction process of polysaccharides was analyzed from principal component analysis(PCA),which in order to explore whether microwave extraction affected structural characteristics of the polysaccharide product.Monosaccharide composition of Artemisia sphaerocephala polysaccharides(ASP)was detected,which were obtained under different microwave extraction conditions.It was showed that ASP was composed of L-arabinose,D-xylose,D-lyxose,D-mannose,D-glucose and D-galactose(molar ratio of 1∶4.98∶1.69∶27.86∶3.76∶13.92),of which mannose and galactose were the main monosaccharides.The result indicated that the change of microwave extraction conditions had an obvious effect on the content of main monosaccharide in ASP.Meanwhile,microwave extraction time and temperature were positively correlated with main monosaccharide content,while microwave extraction power was opposite.Among them,the effect of microwave extraction time was the most significant,and the optimum microwave extraction conditions were 30~60 min,400~600 W and 50~70℃.In summary,the present study provided data support and basis for microwave extraction in the utilization of biomass resources.

关 键 词：主成分分析 圆头蒿多糖 微波提取 单糖组成 

分 类 号：TS201.1[轻工技术与工程—食品科学]