高良姜素对肝癌细胞HepG 2的凋亡效应  

Apoptotic Effects of Galangin on the Hepatoma Carcinoma Cells HepG-2

作　　者：刘政 王会 LIU Zheng;WANG Hui(College of Food Science and Engineering,Jinzhou Medical University,Jinzhou 121001,China)

机构地区：[1]锦州医科大学食品科学与工程学院,辽宁锦州121001

出　　处：《食品工业科技》2020年第22期299-304,共6页Science and Technology of Food Industry

基　　金：辽宁省自然科学基金(20180550694);科技部国家科技基础条件平台课题(TZDWZYK2019-11)。

摘　　要：以人肝癌细胞HepG-2为研究对象,探讨高良姜素对肝癌细胞增殖及凋亡的影响。研究不同浓度的高良姜素对HepG-2细胞毒性及形态学、凋亡率、细胞周期及线粒体膜电位、胞内钙离子稳态的影响。结果表明:5.4、10.8和21.6μg/mL高良姜素使细胞增殖受到抑制,48 h光密度(optical density,OD)值分别为1.295、1.170、1.043,呈现典型的凋亡形态学变化,细胞周期阻滞于DNA合成前期(first gap,G1)。出现凋亡细胞且凋亡率均呈浓度和时间依赖性,48 h凋亡率分别为23.34%、33.15%、44.15%。细胞线粒体膜电位降低,空白对照细胞线粒体膜电位相对荧光强度为27.32,5.4、10.8和21.6μg/mL高良姜素处理组分别为11.26、7.23、3.17;细胞内钙离子浓度增大,空白对照胞内钙离子浓度相对荧光强度为3.82,5.4、10.8和21.6μg/mL高良姜素处理组分别为6.83、11.63、18.73;胞内钙离子稳态被打破。高良姜素可通过阻滞细胞周期进程,降低线粒体膜电位,打破细胞内钙离子稳态来诱导肝癌细胞HepG-2凋亡。To investigate proliferative and apoptotic effects of galangin on hepatoma carcinoma cells,hepatoma carcinoma cells HepG-2 was used as research materials.Different concentrations of galangin affect on the cytotoxicity,morphology,apoptosis rate,cell cycle,mitochondrial membrane potential and intracellular calcium homeostasis of HepG-2 were analyzed.Results showed that Cell proliferation was inhibited by 5.4,10.8 and 21.6μg/mL galangin,OD values were 1.295,1.170,1.043 after treatment for 48 h,respectively.Cells appeared typical apoptosis morphological alterations.Apoptotic cells appeared and the apoptosis rate showed a dosage and duration dependent manner,48 h apoptotic rates were 23.34%,33.15%,44.15%,respectively.Cell cycle was arrested at G1 phase,mitochondrial trans-membrane potential decreased,the relative fluorescence intensity of the mitochondrial membrane potential of blank control cells was 27.32,5.4,10.8 and 21.6μg/mL treatment group were 11.26,7.23 and 3.17,respectively.Intracellular free Ca2+increased,the relative fluorescence intensity of the intracellular Ca2+concentration was 3.82,5.4,10.8 and 21.6μg/mL treatment group were 6.83,11.63 and 18.73,respectively.Galangin induced apoptosis of HepG-2 by affecting cell cycle progression,reducing mitochondrial trans-membrane potential and disturbing intracellular calcium homeostasis.

关 键 词：高良姜素 细胞凋亡 肝癌细胞 增殖 

分 类 号：TS201.4[轻工技术与工程—食品科学]