非标记定量蛋白质组学分析冻结方式对中华管鞭虾肌肉差异蛋白质的影响  

Effect of Freezing Methods on Muscular Differential Protein of Solenocera melantho Based on the Analysis of Label-Free Quantitative Proteomics

作　　者：石娟 李勇勇 周丽萍[1] 李超群[1] 王晴[1] 唐道军[2] 娄永江[1] SHI Juan;LI Yongyong;ZHOU Liping;LI Chaoqun;WANG Qing;TANG Daojun;LOU Yongjiang(College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo,Zhejiang 315211;School of Marine Science,Ningbo University,Ningbo,Zhejiang 315211)

机构地区：[1]宁波大学食品与药学学院,浙江宁波315211 [2]宁波大学海洋学院,浙江宁波315211

出　　处：《核农学报》2020年第12期2681-2691,共11页Journal of Nuclear Agricultural Sciences

基　　金：生鲜农产品储运物流关键技术研究—海捕虾一线保鲜与冷链物流技术研究及产业化示范(2019C02086)。

摘　　要：为了探究冻结方式对中华管鞭虾(Solenocera melantho)肌肉差异蛋白质的影响,本试验运用非标记(label-free)定量蛋白质组学技术,研究液体浸渍冻结、真空包装结合浸渍冻结、静止空气冻结、真空包装结合空气冻结4种方式对中华管鞭虾差异蛋白表达的影响。结果表明,与新鲜中华管鞭虾相比,上述4种冻结方式处理后的虾肌肉中分别有269、162、299、289个差异蛋白,其中表达上调的蛋白分别有100、59、63、61个,表达下调的蛋白分别有169、103、236、228个。筛选出甘油-3-磷酸脱氢酶(NAD^+)、β-胸腺素2个差异蛋白,有望成为与冻结中华管鞭虾品质变化相关的指示蛋白标志物。本研究为深入了解中华管鞭虾在不同冻结方式下的品质变化机制,以及寻找出与新鲜度相关的指示蛋白提供了理论依据,也为虾的冻结工艺技术提供了参考。In order to investigate the effect of freezing methods on muscular differential proteins of Solenocera melantho,the technology of label-free quantitative proteomics was used to study,the expression of differential proteins in Solenocera melantho treated by four freezing methods,including liquid immersion freezing,vacuum packaging combined with immersion freezing,still air freezing,and vacuum packaging combined with air freezing.The results showed that compared with fresh Solenocera melantho there were 269,162,299 and 289 differential proteins in shrimp muscles treated by four freezing methods,respectively.Among them,there were 100,59,63 and 61 proteins was up-regulated and 169,103,236 and 228 proteins was down-regulated.Two differential proteins,glycerol-3-phosphate dehydrogenase(NAD^+)and thymosin beta,were screened to be the expected indicator protein markers related to frozen quality of Solenocera melantho.This study provides a theoretical basis for in-depth understanding of the quality change mechanism of Solenocera melantho under different freezing treatment and for finding indicator proteins related to freshness,and also provides a reference for the improving freezing process technology.

关 键 词：非标记定量蛋白质组学 中华管鞭虾 冻结方式 差异蛋白 生物信息学分析 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]