发酵用高温放线菌的筛选及性质研究  

Screening and properties of high-temperature actinomycetes for fermentation

作　　者：魏越波 郭晓军[1] 孙悦龙 朱宝成[1] Wei Yuebo;Guo Xiaojun;Sun Yuelong;Zhu Baocheng

机构地区：[1]河北农业大学生命科学学院,河北保定071000

出　　处：《饲料工业》2020年第21期47-52,共6页Feed Industry

基　　金：保定市科学技术研究与发展计划项目[15ZN017]。

摘　　要：为了获得一株发酵用耐高温产酶放线菌菌株,试验以秸秆、粪便、菌渣等物料自然腐熟的高温期堆体中央物料为样本进行筛选。首先涂布高氏一号培养基,在高温条件下约50℃左右培养进行菌株的初筛,然后在蛋白酶、淀粉酶和纤维素酶平板上十字划线进行菌株的复筛,将三个酶活均较高的菌株进行种属鉴定,最后研究了菌株在不同温度、不同pH值、不同水分的固体物料上的生长情况。结果表明,初筛获得了35株菌可以在高氏一号培养基60℃生长良好,复筛得到1株3个酶活均较高的菌株Fx-1,通过菌落形态、菌体特征、16S rDNA序列分析和生理生化试验鉴定该菌株为嗜热链球菌(Streptococcus thermophilus)。该菌株可耐60℃左右的高温,最适生长温度是60℃;菌株在pH值6.0~8.0的中性范围内都可以生长,其中在pH值7.0生长最好;菌株在含水量为30%、40%、50%、60%的物料中都能正常生长,在40%含水量条件下菌株生长最好。综上所述,说明本试验获得了1株性质优良的产多种代谢酶的高温放线菌,为饲料发酵提供了优质的菌种资源。To obtain a strain of high temperature resistant enzyme-producing actinomycete,this experiment uses the central material of the high-temperature pile body which is naturally decomposed by straw,manure and slag as a sample for screening.First,the Gaussian No.1 medium is coated,and the strain is firstly sieved at about 50℃under high temperature conditions,and then the strain is rescreened on the protease,amylase and cellulase plates,and the three enzymes are sieved.The strains with higher vigor were identified by species,and finally the growth of strains on solid materials with different temperatures,different pH values and different moisture was studied.The results showed that 35 strains of the obtained strains could grow well in Gao's No.1 medium at 60℃,and a strain of three strains with high enzyme activity,Fx-1,was obtained by rescreening,and the colony morphology,bacterial characteristics,16S were obtained.The rDNA sequence analysis and physiological and biochemical tests identified the strain asStreptococcus thermophilus.The strain can withstand a high temperature of about 60℃,and the optimum growth temperature is 60℃;the strain can grow in the neutral range of pH 6.0~8.0,wherein the growth is best at pH 7.0;the strain has a water content of 30%,40%,50%,60%of the material can grow normally,and the strain grows best under the condition of 40%water content.In summary,it is shown that a high-temperature actinomycete producing a variety of metabolic enzymes was obtained in this experiment,which provided high-quality strain resources for feed fermentation.

关 键 词：发酵 高温放线菌 筛选 生理生化 性质研究 鉴定 

分 类 号：S816.32[农业科学—饲料科学]