石韦辐照保藏技术及抑菌活性评价研究  

Research on Irradiation Preservation Technology and Antimicrobial Activity Evaluation of Pyrrosia petiolosa

作　　者：周洁 吴萌萌 张安东 晏仕英 赵淼淼 吴道艳 黄敏[2] 赵建[1] ZHOU Jie;WU Meng-meng;ZHANG An-dong;YAN Shi-ying;ZHAO Miao-miao;WU Dao-yan;HUANG Min;ZHAO Jian(Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry,College ofLife Sciences,Sichuan University,Chengdu,610064,China;Irradiation Preservation Technology Key Labora tory of Sichuan Province,Sichuan Institute of Atomic Energy,Chengdu 610101,China)

机构地区：[1]四川大学生命科学学院资源微生物及微生物生物技术重点实验室,四川成都610064 [2]四川省原子能研究院辐照保藏四川省重点实验室,四川成都610101

出　　处：《时珍国医国药》2020年第6期1454-1457,共4页Lishizhen Medicine and Materia Medica Research

基　　金：四川省重点研发项目(2017FZ0059);四川省原子能研究院辐照保藏四川省重点实验室开放基金资助项目(FZBC2018003)。

摘　　要：目的研究辐照应用于石韦鲜药保鲜的可行性。方法采用0,3,6,9,12kGy剂量的60Co-γ射线辐照处理鲜石韦后,不同保藏条件下分析不同辐照条件对石韦原料保鲜和抑菌活性的影响。结果与空白相比,辐照处理可有效杀灭石韦上的微生物,并确定石韦的最佳辐照剂量为9kGy、常温条件下保藏1~30d石韦抑菌活性稳定。结论辐照技术有利于石韦鲜药的运输且不产生霉变,对石韦鲜药资源利用率的提升和广泛应用奠定了基础。Objective To study the feasibility of gamma-ray irradiation on the preservation of fresh herb of Pyrrosia petiolosa.Methods Fresh Pyrrosia petiolosa irradiated with 60Co-γradiation at doses of 0,3,6,9,12 kGy,and analyzed the effects of different irradiation conditions on the preservation and antibacterial activity of Pyrrosia petiolosa fresh materials under different preservation conditions.Results Compared with the blank,the irradiation technology can effectively kill microorganisms on Pyrrosia petiolosa,and the optimal irradiation dose of Pyrrosia petiolosa is 9 kGy,and the antibacterial activity of Pyrrosia petiolosa preserved at normal temperature for 1 to 30 days is stable.Conclusion The 60Co-γ-ray irradiation technology is beneficial to the transportation of fresh materials of Pyrrosia petiolosa and does not generate mildew,thus laying a foundation for improving the utilization rate of Pyrrosia petiolosa resources and wide application.

关 键 词：石韦 辐照保藏 酯提物 抑菌活性 气相色谱-质谱法 

分 类 号：R288[医药卫生—中药学] R285.5[医药卫生—中医学]