肝细胞生长因子受体蛋白表达及基因突变与胃癌临床病理特征的关系  

The relationship between c-Met protein expression and gene mutations and clinicopathological characteristics of gastric carcinoma

作　　者：宗桂娟[1] 尹海兵[1] 李春笋[1] 陈旭东[1] 陶玉[1] 杨磊[2] 赵斌[1] 何松[1] ZONG Guijuan;YIN Haibing;LI Chunsun;CHEN Xudong;TAO Yu;YANG Lei;ZHAO Bin;HE Song(Department of Pathology,Nantong Tumor Hospital,Nantong,Jiangsu 226361,China;Department of Oncology,Nantong Tumor Hospital,Nantong,Jiangsu 226361,China)

机构地区：[1]南通市肿瘤医院病理科,江苏南通226361 [2]南通市肿瘤医院肿瘤内科,江苏南通226361

出　　处：《安徽医药》2020年第11期2174-2177,共4页Anhui Medical and Pharmaceutical Journal

基　　金：南通市科技项目(HS2015003)。

摘　　要：目的探讨肝细胞生长因子受体(c-Met)蛋白表达及基因突变与胃癌临床病理特征的关系及对胃癌发生发展的影响。方法选取2016年1月至2017年12月南通市肿瘤医院胃癌病人246例。对手术切除的胃癌标本采用免疫组织化学法及荧光定量PCR法检测c-Met蛋白表达水平和基因突变状态。结果c-Met蛋白表达阳性率为83.3%,c-Met蛋白表达与病人性别、年龄、肿瘤大小、分化程度、淋巴结转移、临床分期无明显关系(P>0.05);与脉管侵犯有关(P<0.05);c-Met基因突变率与蛋白表达检出率差异有统计学意义(P<0.05)。结论c-Met蛋白表达与脉管侵袭有关;不同性别、年龄、组织分化之间的c-Met基因突变率差异不大,且c-Met基因突变率与蛋白表达不一致。Objective To investigate the relationship between c-Met protein expression and and gene mutations and clinicopathological features of gastric carcinoma,and their influence on the occurrence and development of gastric carcinoma.Methods A total of 246 patients with gastric cancer in Nantong Cancer Hospital from January 2016 to December 2017 were selected.The expression of c-Met protein and gene mutation status were detected by immunohistochemistry and real-time PCR on the surgically resected gastric cancer specimens.Results The expression of c-Met protein was not associated with gender,age,tumor size,degree of differentiation,clinical stage,and lymph node metastasis(P>0.05);it was associated with vascular invasion(P<0.05);c-Met gene mutation rate and protein expression test There was a statistically significant difference in the rate of outflow(P<0.05).Conclusion The expression of c-Met protein is related to vascular invasion.There is no significant difference in the mutation rate between c-Met gene in different genders,ages and tissue differentiation,and the mutation rate of c-Met gene is inconsistent with protein expression.

关 键 词：胃肿瘤/病因学 原癌基因蛋白质C-MET 肿瘤侵润 基因突变 

分 类 号：R735.2[医药卫生—肿瘤]