基于RNA-Seq挖掘玫瑰冠鸡与科宝鸡胚胎期胸肌组织差异表达基因  

Identification of differentially expressed genes in embryonic breast muscle tissue of Rose-crowned chicken and Cobb broilers based on RNA-Seq

作　　者：张蕾[1] 任嵩[1] 杨娴婧 孙杰[1] 廖和荣[1] ZHANG Lei;REN Song;YANG Xian-jing;SUN Jie;LIAO He-rong(College of Animal Science and Technology,Shihezi University,Shihezi 832000,China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832000

出　　处：《江苏农业学报》2020年第5期1237-1246,共10页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31660654、31860641)。

摘　　要：本研究旨在挖掘玫瑰冠鸡与科宝鸡胚胎期胸肌组织中差异表达基因,探讨2种鸡肌肉发育及肌内脂肪(IMF)沉积的分子遗传机理。本研究利用RNA-Seq技术对玫瑰冠鸡和科宝鸡胚胎期胸肌组织中差异表达基因(Differentially expressed genes,DEGs)进行筛选,通过GO富集和KEGG数据库对DEGs的功能进行注释与分析。结果显示:4个文库共鉴定到4643个差异表达基因,其中玫瑰冠公鸡胸肌和科宝公鸡胸肌中有1909个显著表达的基因,以科宝鸡为参照,929个上调,980个下调;玫瑰冠母鸡胸肌和科宝母鸡胸肌中有2734个显著表达的基因,其中1444个上调,1290个下调。经荧光定量PCR验证,选取的12个差异基因的表达趋势与测序结果一致。GO富集结果表明,MYL2调控骨骼肌的生长发育,MYOG参与肌细胞的形成与分化;ACACA、ACOX2和ACOX3参与脂肪酸代谢过程;Notch1参与了胚胎发育及脂肪分化相关过程。此外,KEGG通路分析结果显示,能量代谢相关的信号通路(mTOR)与肌肉发育相关的信号通路相互作用影响肌纤维的发育。细胞连接相关通路(局部粘附、ECM-受体互作)参与了肌肉脂肪代谢过程。本研究结果为进一步解析鸡优质肉质性状相关的遗传机制奠定了基础。This study aimed to identify differentially expressed genes(DEGs)in the embryonic breast muscle tissue of Rose-crowned chicken and Cobb broilers,and to explore the molecular genetic mechanism in muscle development and intramuscular fat(IMF)deposition.RNA-Seq technology was used to screen differentially expressed genes in the embryonic breast muscle tissues of Rose-crowned chicken and Cobb broilers.GO enrichment and KEGG database were used to annotate the functions of DEGs.The results showed that a total of 4643 differentially expressed genes were identified in the four libraries.Among them,1909 genes were significantly expressed in the breast muscle of Rose-crowned cocks and Cobb cocks.Taking Cobb broilers as reference,929 genes were up-regulated and 980 genes were down-regulated.There were 2734 significantly expressed genes in the breast muscle of Rose-crouned henes and Cobb hens,1444 genes were up-regulated and 1290 genes were down-regulated.The results of real-time PCR indicated that the expression trend of 12 differential genes was consistent with the sequencing results.Go enrichment analysis results showed that MYL2 regulated the growth and development of skeletal muscle.MYOG was involved in the formation and differentiation of muscle cells.ACACA,ACOX2 and ACOX3 were involved in the fatty acid metabolism.Notch1 was involved in embryo development and adipogenesis-related processes.In addition,KEGG pathway analysis results indicated that the interaction between the energy metabolism-related signaling pathway(mTOR)and muscle development-related signaling pathways affected the development of muscle fibers.Cell junction-related pathways(focal adhesion,ECM-receptor interaction)were involved in IMF metabolism.The results of this study lay the foundation for further analysis of genetic mechanisms associated with high-quality meat quality traits in chickens.

关 键 词：玫瑰冠鸡 科宝鸡 胚胎期 胸肌 差异表达基因 RNA-SEQ 

分 类 号：S831.2[农业科学—畜牧学]