超高效液相色谱-串联质谱法测定毒蘑菇中5种强毒性蘑菇毒素含量  

Determination of 5 kinds of virulent mushroom toxins in poisonous mushroom by ultra performance liquid chromatography tandem mass spectrometry

作　　者：许欣欣[1] 陈春晓[1] 仲岳桐[1] 陈慧玲[1] 吕昭颖 姜杰[1] XU Xin-Xin;CHEN Chun-Xiao;ZHONG Yue-Tong;CHEN Hui-Ling;LV Zhao-Ying;JIANG Jie(Shenzhen Center for Disease Control&Prevention,Shenzhen 518055,China)

机构地区：[1]深圳市疾病预防控制中心,深圳518055

出　　处：《食品安全质量检测学报》2020年第19期6936-6941,共6页Journal of Food Safety and Quality

基　　金：深圳市三名工程项目(SZSM201811070)。

摘　　要：目的建立超高效液相色谱-串联质谱法快速检测毒蘑菇中α-鹅膏毒肽、β-鹅膏毒肽、γ-鹅膏毒肽、羧基二羟鬼笔毒肽和二羟鬼笔毒肽5种强毒性蘑菇毒素含量的分析方法。方法样品经甲醇/水(3:7, V:V)混合液超声提取,离心后取上清液过0.22μm微孔滤膜,C18色谱柱分离,超高效液相色谱-串联质谱仪上采用多反应监测正离子模式测定,外标法定量检测。结果蘑菇样本中检测出α-鹅膏毒肽、β-鹅膏毒肽、γ-鹅膏毒肽和羧基二羟鬼笔毒肽4种蘑菇毒素,浓度分别为164、198、33、2208 mg/kg。结论本方法快速简便、准确,可用于因蘑菇毒素引起的食物中毒应急检测。Objective To establish a method for rapid determination of 5 highly toxic mushroom toxins including α-amanitin, β-amanitin, γ-amanitin, phallacidin, phalloidin in poisonous mushrooms by ultra performance liquid chromatography-tandem mass spectrometry. Methods Ultrasonic extraction of methanol/water(3:7, V:V) mixture was performed. After centrifugation, the supernatant was separated by 0.22 μm microporous filtration membrane, and separated by C18 chromatographic column. It was determined by multi-reaction monitoring positive ion mode using ultra performance liquid chromatography-tandem mass spectrometer, and external standard method for quantitative detection. Results Four kinds of mushroom toxins were detected in the mushroom sample, namely, α-amanitin, β-amanitin, γ-amanitin, and phallacidin, with concentrations of 164, 198, 33, and 2208 mg/kg, respectively. Conclusion This method is rapid, simple and accurate, and is suitable for emergency detection of food poisoning caused by mushroom toxin.

关 键 词：超高效液相色谱-串联质谱法 蘑菇毒素 鹅膏毒肽 鬼笔毒肽 

分 类 号：R155.3[医药卫生—营养与食品卫生学] O657.63[医药卫生—公共卫生与预防医学]