菟丝子多糖的抗氧化活性和抑制肿瘤细胞增殖的研究  

Study on the antioxidant activity and the inhibition of tumor cell proliferation of polysaccharides from the seeds of Cuscuta chinensis Lam.

作　　者：叶春林 KHUDOYBERDIEV Ilkhomjon 陈颖 李瀚鑫 YE Chunlin;KHUDOYBERDIEV Ilkhomjon;CHEN Ying;LI Hanxin(School of Biological and Chemical Engineering,Zhejiang Provincial Key Laboratory of Chemical&Biological Processing Technology of Farm Produces,Zhejiang University of Science and Technology,Hangzhou 310023,China)

机构地区：[1]浙江科技学院生物与化学工程学院,浙江省农产品化学与生物加工技术重点实验室,浙江杭州310023

出　　处：《河南工业大学学报（自然科学版）》2020年第5期73-78,共6页Journal of Henan University of Technology：Natural Science Edition

基　　金：浙江省大学生科技创新活动计划暨新苗人才计划“菟丝子抗肿瘤有效成分的筛选”(2018R415017)。

摘　　要：探讨菟丝子多糖的抗氧化活性和抑制肿瘤细胞的增殖作用可为其开发利用提供依据。通过测定菟丝子多糖对DPPH自由基、羟自由基、超氧阴离子自由基的清除率和总还原力,研究其抗氧化活性,并用MTT法检测其对人肺腺癌细胞A549、人肝癌细胞HepG2、人胰腺癌细胞PANC-1和人正常肝细胞L-02、人胚肺成纤维细胞HFL-1增殖的抑制作用。结果表明:菟丝子多糖清除DPPH自由基、羟自由基和超氧阴离子自由基的IC 50分别为0.25、0.36、0.18 mg/mL;在1.0 mg/mL时,还原力(OD 700)为0.55。菟丝子多糖抑制A549、HepG2和PANC-1细胞的IC 50分别为137.6、341.7、625.4μg/mL。菟丝子多糖对L-02和HFL-1毒性极小,IC 50分别为1.223、1.299 mg/mL。在肿瘤细胞A549、HepG2和PANC-1的半数抑制浓度时,正常细胞L-02和HFL-1的成活率在90%以上,表明菟丝子多糖可以选择性抑制肿瘤细胞的生长,而对正常细胞增殖抑制作用很小。This study investigated the antioxidant activity and the inhibition effect of tumor cell proliferation of polysaccharides from the seeds of Cuscuta chinensis Lam.The scavenging rates of the polysaccharides on DPPH,hydroxyl and superoxide anion free radicals and total reducing power were evaluated.The inhibitory effect of the polysaccharides on the proliferation of A549,HepG2,PANC-1 tumor cells and human normal liver cell L-02,human normal fetal lung fibroblast HFL-1 cells were determined by MTT assay.Results showed that the IC 50 values of the polysaccharides for scavenging DPPH,hydroxyl,and superoxide radicals were 0.25 mg/mL,0.36 mg/mL,and 0.18 mg/mL,respectively.The reducing power was 0.55(OD 700),when the concentration of polysaccharides was 1.0 mg/mL.The IC 50 values on cytotoxicity were 137.6μg/mL,341.7μg/mL,and 625.4μg/mL for A549,HepG2,and PANC-1 cells,respectively.The polysaccharides were almost nontoxic to L-02 and HFL-1 cells as their IC 50 values(1.223 and 1.299 mg/mL for L-02 and HFL-1 cells,respectively)were much higher.At the 50%inhibitory concentration of A549,HepG2,and PANC-1 tumor cells,the survival rate of normal hepatocyte L-02 and HFL-1 was more than 90%,indicating that the polysaccharide could selectively inhibit the growth of tumor cells and rarely inhibit the growth of normal cells.

关 键 词：菟丝子 多糖 抗氧化活性 抗肿瘤 

分 类 号：TS201.2[轻工技术与工程—食品科学]