砷氧化菌株的筛选及Bosea sp.AS-1基因组分析  

Screening and genome analysis of arsenite-oxidizing strain Bosea sp.AS-1

作　　者：柳朝阳 向利 王红梅[1,2] 鲁小璐[1] Chaoyang Liu;Li Xiang;Hongmei Wang;Xiaolu Lu(School of Environmental Studies,China University of Geosciences(Wuhan),Wuhan 430074,Hubei Province,China;State Key Laboratory of Biogeology and Environmental Geology,China University of Geosciences(Wuhan),Wuhan 430074,Hubei Province,China)

机构地区：[1]中国地质大学(武汉)环境学院,湖北武汉430074 [2]中国地质大学(武汉)生物地质与环境地质国家重点实验室,湖北武汉430074

出　　处：《微生物学报》2020年第11期2538-2554,共17页Acta Microbiologica Sinica

基　　金：国家自然科学基金(41877320,41402300)。

摘　　要：【目的】对湖南省锡矿山地区的砷氧化菌株的种属进行初步鉴定,并对砷锑氧化菌株Bosea sp. AS-1 (简称AS-1)进行全基因组测序和生物信息学分析。【方法】分离砷氧化菌株,并利用16S rRNA基因测序进行菌种鉴定。在此基础上,对能够高效氧化砷的菌株AS-1进行全基因组测序,对测序数据进行基因组装和功能注释、COG、GO及KEGG聚类分析,以及次级代谢产物合成基因簇与代谢途径预测等。【结果】湖南省锡矿山的砷氧化菌株主要分布在α-、β-、γ-变形菌纲以及厚壁菌门。菌株AS-1基因组的测序结果显示AS-1基因组包含一条大小为5.536Mb环状染色体和两个大小分别为189.9kb和112.1kb的质粒。对AS-1基因组进一步分析发现该菌株的基因组中包含砷锑代谢相关基因,还有鞭毛形成、鞭毛运动及生物膜形成的基因,这些基因的存在可能与AS-1能高效耐受和氧化砷和锑的特性相关。此外,菌株AS-1中还存在部分碳固定基因和硫氧化基因,这暗示着AS-1能够进行自养生长并氧化环境中的硫元素。【结论】菌株AS-1可以在自养条件下生长并且能够氧化Sb(Ⅲ)为Sb(Ⅴ)。[Objective]In this study,we aimed to characterize the community structure of arsenite-oxidizing bacteria in Xikuangshan of Hunan Province,and the whole genome of an arsenite-antimonite-oxidizing strain Bosea sp.AS-1(Abbreviation:AS-1).[Methods]Arsenite-oxidizing bacterial strains were isolated from the samples collected in Xikuangshan,and 16S rRNA genes were sequenced for phylogenic analysis.Whole genome of strain AS-1 was sequenced and analyzed using relevant software and databases for genome assembly,gene prediction and functional annotation.[Results]The arsenite-oxidizing bacteria in Xikuangshan were mainly distributed inα-,β-,γ-Proteobacteria and Firmicutes.AS-1’s genome contained one circular chromosome with a size of 5.536 Mb and two plasmids of 189.9 kb and 112.1 kb,respectively.Further analysis on AS-1’s genomic data reveal many genes related to arsenic and antimony metabolism,as well as flagella formation,flagellar movement,and biofilm formation.These genes may be involved in AS-1’s resistance to high level of arsenic and antimony in environment.Besides,several carbon-fixation genes and sulfur-oxidizing genes were also found in the genome of AS-1,suggesting that AS-1 may grow autotrophically and oxidize sulfur.[Conclusion]Subsequent experiments confirmed autotrophic growth of AS-1.

关 键 词：Bosea sp. AS-1 全基因组测序 砷、锑代谢基因 代谢通路 

分 类 号：X172[环境科学与工程—环境科学]