一种来源嗜热古菌的耐热β-葡萄糖苷酶制备鸢尾苷元的工艺研究  

Study on the Enzymatic Hydrolysis Process of tectorgenin by a Thermostableβ-glucosidase from Pyrococcus Furiosus

作　　者：王仲 方伟 董青 李丽娜 Wang Zhong;Fang Wei;Dong Qing;Li Li’na(Wuhan Huaxia University of Technology,Wuhan 430223,China;Hubei Biopesticide Engineering Research Center,Wuhan 430064,China)

机构地区：[1]武汉华夏理工学院,湖北武汉430223 [2]湖北省生物农药工程研究中心,湖北武汉430064

出　　处：《山东化工》2020年第21期44-46,共3页Shandong Chemical Industry

基　　金：湖北省农业科技创新项目(2019-620-000-001-27)。

摘　　要：目的:研究应用一种来源嗜热古菌的β-葡萄糖苷酶制备鸢尾苷元的最佳酶解工艺。方法:本实验以鸢尾黄素-7-O-木糖葡萄糖苷作为底物,用耐热β-葡萄糖苷酶,在单因素试验的基础上,采用响应面分析法优化最佳酶解条件。结果:最佳工艺条件为:底物与酶的质量比为1∶5,反应温度85℃,缓冲液pH6.0,反应1h,鸢尾苷元的转化率最高。结论:β-葡萄糖苷酶转化鸢尾苷元完全,转化速率快,且操作简单方便,适合工业推广。Objective:Study on the optimization of enzymatic hydrolysis process of tectorgenin by aβ-glucosidase.Methods:The tectorigenin-7-O-xylosylglucoside was obtained as the substrate by extraction,isolation and refining.Based on the single-factor tests,the response surface method was used to optimize the enzymatic hydrolysis conditions.Results:The optimum enzymatic hydrolysis conditions were as follows:substrate to enzyme ratio 1∶5,reaction temperature 85℃,time 1h,and pH6.0.Under the optimum conditions,the bioconversion rate of tectorgenin was the maximum.Conclusion:These results suggest that this enzyme can be useful for the industrial hydrolysis of tectorgenin.

关 键 词：鸢尾苷元 Β-葡萄糖苷酶 酶解 响应面法 

分 类 号：TQ28[化学工程—有机化工]