UPLC-MS/MS研究36个梨品种成熟果实中的特征性多酚和三萜酸类物质  

Analysis of Characteristic Polyphenols and Triterpenic Acids in Ripe Pears of 36 Cultivars by UPLC-MS/MS

作　　者：孙莉琼 郝雯菁 唐晓清[1] 王康才[1] 张绍铃[1] SUN Liqiong;HAO Wenjing;TANG Xiaoqing;WANG Kangcai;ZHANG Shaoling(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学园艺学院,江苏南京210095

出　　处：《食品科学》2020年第22期206-214,共9页Food Science

基　　金：国家自然科学基金青年科学基金项目(31801825);中央高校基本科研业务费专项(130Z521911202)。

摘　　要：选择36个代表性梨栽培品种为试材,采用超高效液相色谱-串联质谱的多离子反应监测模式,对梨成熟果中的102个多酚与16个三萜酸类化学成分展开系统分析,并结合前期对幼果的研究,分析成熟后主要成分的变化。结果表明,梨果实中含有丰富的多酚和三萜酸类物质,其中奎尼酸、熊果苷和绿原酸为主要多酚类化合物,熊果酸为主要的三萜酸类化合物,但大部分物质的含量与幼果期相比显著降低。同时通过综合分析发现奎尼酸、绿原酸等12个酚酸化合物,熊果苷等8个酚苷化合物,芦丁和异鼠李素-3-O-芸香糖苷等5个黄酮化合物,儿茶素和表儿茶素等5个黄烷-3-醇化合物,白桦酸脂、齐墩果酸和熊果酸等10个化合物在梨属果实中普遍存在,初步确定为梨属果实的特征性多酚和三萜酸类物质。主成分分析和偏最小二乘-判别分析结果表明不同梨品种果实的化学成分整体差异较小,但秋子梨系统样品的品种特异性较强。该研究结果为梨属果实质量评价体系的进一步完善,以及果实后期各种功能性产品的加工选种提供一定参考.In this work,102 polyphenols and 16 triterpenic acids in ripe pears of 36 cultivars were analyzed by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)with multiple reaction monitoring(MRM)mode,changes in the major components were investigated in comparison with data from our previous research on young fruits.It was found that pear fruits were rich in polyphenols and triterpenoids,among which,chlorogenic acid,quinic acid and arbutin were the primary polyphenols and ursolic acid was the predominant triterpenoid.The contents of most of these compounds were significantly reduced in ripe pears compared with young pears.Generally,12 phenolic acid compounds such as quinic acid and chlorogenic acid,eight phenolic glycosides such as arbutin,five flavonoid compounds such as rutin and isorhammetin-3-O-rutinoside,five flavan-3-ols such as catechin and epicatechin,and 10 triterpenic acids,such as betulinic acid,oleanolic acid and ursolic acid were ubiquitously detected in all cultivars and tentatively identified as the characteristic polyphenolic and triterpenoid constituents.Furthermore,the results of principal component analysis(PCA)and partial least squares-discriminant analysis(PLS-DA)showed that there was no significant difference among various pear cultivars and Pyrus ussuriensis Maxim cultivars exhibited strong species specificity.This study provides reference for further improving the pear quality evaluation system and for cultivar selection for various functional products in the future.

关 键 词：梨 多酚 黄酮 三萜酸 超高效液相色谱-串联质谱 

分 类 号：S661.2[农业科学—果树学]