南极磷虾肽促进小鼠骨折愈合作用及其机制  

Promoting Effect and Mechanism of Peptides from Antarctic Krill on Fracture Healing in Mice

作　　者：李卓 田迎樱 李雪静 于朋[1] 王静凤[1] LI Zhuo;TIAN Yingying;LI Xuejing;YU Peng;WANG Jingfeng(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;Marine Biomedical Research Institute of Qingdao,Qingdao 266000,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]青岛海洋生物医药研究院,山东青岛266000

出　　处：《食品科学》2020年第21期99-105,共7页Food Science

基　　金：“十三五”国家重点研发计划重点专项(2018YFC0311203)。

摘　　要：目的:从南极磷虾粉中酶解分离出南极磷虾肽(peptides from Antarctic krill,AKP),探究其对小鼠骨折愈合的影响及机制。方法:对雌性C57BL/6小鼠进行右侧胫骨开放性骨折手术,术后随机分为骨折模型组、AKP低剂量组(200 mg/kg mb)、AKP高剂量组(400 mg/kg mb)。于小鼠骨折术后5、10、21 d进行取材,动态分析AKP对小鼠血清生化指标、骨痂组织形态学及软骨内骨化关键基因转录的影响。结果:AKP能显著上调血清转化生长因子β(transforming growth factor-β,TGF-β)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、I型胶原蛋白(collagen type I,Col1α)水平(P<0.05);AKP能够剂量依赖性地促进纤维骨痂的形成、软骨性骨痂的形成与转变以及硬骨性骨痂的形成与改建;AKP可显著上调早期成软骨细胞分化因子(SOX9、Col10α)、中后期血管生成基因(VEGF、Ang1)及软骨基质降解因子MMP13和新骨生成相关基因(Col1α、OCN、TGF-β)的mRNA转录水平(P<0.05)。结论:AKP可通过加速软骨内骨化过程从而促进骨折愈合。Objective:Peptides from Antarctic krill(AKP)were isolated from an enzymatic hydrolysate of Antarctic krill powder to explore its effect and underlying mechanism on fracture healing in mice.Methods:Female C57BL/6 mice underwent surgery for open fracture of the right tibia and were randomly divided into model control,low-dose(200 mg/kg mb)and high-dose(400 mg/kg mb)AKP groups.At 5,10 and 21 days after surgery,samples were taken to dynamically analyze the effects of AKP on serum biochemical parameters,epiphyseal morphology and the transcription of the key genes related to endochondral ossification.Results:AKP could significantly increase the concentration of transforming growth factor-β(TGF-β),vascular endothelial growth factor(VEGF)and collagen type I(Col1α)in serum(P<0.05),and promote the formation of fibrous callus,the formation and transformation of cartilage callus and the formation and reconstruction of sclerotic callus in a dose-dependent manner.AKP also significantly up-regulated the mRNA transcription levels of early chondrocyte differentiation factor(SOX9 and Col10α),middle and late angiogenic genes(VEGF and Ang1),cartilage matrix degradation factor(MMP13)and bone formation-related genes(Col1α,OCN and TGF-β).Conclusion:AKP can promote fracture healing by accelerating the process of endochondral ossification.

关 键 词：南极磷虾肽 骨折愈合 组织形态学 软骨内骨化 

分 类 号：TS201.4[轻工技术与工程—食品科学]