鸡胚蛋白的酶解工艺优化及抗氧化性研究  

Antioxidant and Optimization of Enzymatic Hydrolysis of Chicken Embryo Protein

作　　者：付劢 陈继兰[2] 王攀林 张瑛毓 王凤忠[1] 张丽静[1] FU Mai;CHEN Jilan;WANG Panlin;ZHANG Yingyu;WANG Fengzhong;ZHANG Lijing(Institute of Agro-Products Processing Chinese Academy of Agricultural Sciences,Beijing 100193,China;Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]中国农业科学院农产品加工研究所,北京100193 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《食品科技》2020年第9期35-42,共8页Food Science and Technology

基　　金：中国农业科学院农产品加工研究所创新工程项目(125161015000150013)。

摘　　要：为进一步开发鸡胚蛋的食用价值与保健功能,以鸡胚蛋为原料,酶解时间、酶解温度、pH、酶与底物比为响应因素,水解度为指标,采用响应面分析法优化鸡胚多肽的提取工艺。在单因素实验的基础上通过Box-Behnken响应面分析法研究分析比较了枯草杆菌蛋白酶、碱性蛋白酶、木瓜蛋白酶、菠萝蛋白酶、胰蛋白酶在内的5种蛋白酶的酶解效果,并通过DPPH·清除率评价获得的鸡胚多肽的抗氧化性。结果表明,在pH为7、酶与底物比3%:1、酶解温度55℃的条件下,用菠萝蛋白酶水解6 h水解度最高,酶解产物具有一定的抗氧化活性。研究结果为鸡胚作为新食品原料的开发利用提供了理论依据与方法。In order to explore the value and health benefits of chicken embryonated egg,we used embryonated egg as raw materials,and the parameters of enzymatic hydrolysis of peptides from embryonated egg were optimized by response surface analysis.The degree of hydrolysis was the index to evaluate the difference of chicken embryonated egg hydrolyzed.The enzymatic hydrolysis time,enzymatic hydrolysis temperature,pH,enzyme to substrate ratio were the response factors.Based on the single factor experiments,five proteases including subtilisin,alkaline protease,papain,bromelain and trypsin were used to analyze the enzymolysis effect of chicken embryos by Box-Behnken response surface analysis to explore the best process conditions to obtain the active polypeptide.And the antioxidant capacity of chicken embryo polypeptide was evaluated by DPPH clearance rate.The results indicated that the highest hydrolysis degree conditions obtained are as follows:pH is 7,enzyme to substrate ratio is 3%:1,enzymatic hydrolysis temperature is 55℃,enzymatic hydrolysis time is 6 h by bromelain.Verification experiments were carried out under the optimum extraction.The results of this study provided a certain theoretical basis for the development and utilization of embryonated egg.

关 键 词：鸡胚蛋 蛋白酶 响应面分析 抗氧化性 

分 类 号：TS253.1[轻工技术与工程—农产品加工及贮藏工程]