一起由两种罕见血清型副溶血性弧菌引起食物中毒事件分析  

Etiological analysis of a case of food poisoning caused by mixed infection with two rare serotypes of Vibrio parahaemolyticus

作　　者：黄贤[1] 何冬梅[2] 李振翠 李永青[1] 林思灵 HUANG Xian;HE Dongmei;LI Zhencui;LI Yongqing;LIN Siling(Longmen County Center for Disease Control and Prevention,Huizhou,Guangdong 516800,China;Guangdong Provincial Center for Disease Control and Prevention,Guangzhou,Guangdong 511430,China)

机构地区：[1]龙门县疾病预防控制中心,广东惠州516800 [2]广东省疾病预防控制中心,广东广州511430

出　　处：《中国热带医学》2020年第11期1112-1115,共4页China Tropical Medicine

摘　　要：目的分析一起由两种罕见血清型混合感染引起的副溶血性弧菌食物中毒病原学的检测结果,为食物中毒病原学检查途径提供依据。方法对一起在龙门县人民医院就诊食物中毒事件的9例患者采集其肛拭子样本,参照《食品微生物学检验副溶血性弧菌检验》(GB 4789.7—2013)等标准进行病原学分析鉴定。结果本次事件出现临床症状者9例,其中≤20岁2例,20~<40岁1例,40~<60岁4例,≥60岁2例;9份肛拭子样本检出8株副溶血性弧菌,血清分型O2:K28构成比占25.00%(2/8);O8:K21构成比占75.00%(6/8);经PFGE分子分型,2株O2∶K28血清型(VP18171、VP18175)相似度为66.7%;3株O8∶K21血清型(VP18172、VP18173、VP18176)相似度为100.0%,与另外3株O8∶K21血清型(VP18177、VP18174、VP18170)相似度为88.4%~95.2%;药敏试验结果显示,除有1株菌株对氨苄西林、头孢唑林二重耐药、1株菌株对氨苄西林耐药、3株菌株对头孢唑林耐药外,其它菌株对所有测试药物均敏感,无多重耐药现象。结论此次食物中毒是由O2:K28和O8:K21这两种罕见血清型的副溶血性弧菌混合感染引起的。Objective To analyze the etiology of a food poisoning caused by mixed infection with two rare serotypes of Vibrio parahaemolyticus,and we provide the basis for the pathogen examination of food poisoning.Methods According to Food Microbiology Testing for Vibrio parahaemolyticus(GB 4789.7-2013) and other standards,9 patients with food poisoning treated in Longmen County People’ s Hospital were selected as research objects,and their rectal swabs were collected for analysis and identification.Results There were a total of 9 patients with clinical symptoms,including 2 patients aged≤20 years old,1 patient aged 21-<40 years old,4 patients aged 41-<60 years old,and 2 patients aged≥60 years old.Eight strains of Vibrio parahaemolyticus were detected in 9 rectal swabs,with ratios of the blood classification type O2:K28 was 25.00%(2/8),and O8:K21 was:75.00%(6/8);The similarity of O2:K28 serotypes(VP18171 and VP18175) was 66.1% by PFGE molecular typing.The similarity of O8:K21 serotype(VP18172,VP18173,VP18176) was 100.0%,and the similarity of 08:K21 serotype(VP18177,VP18174,VP18170) was 88.4%-95.2%.The results of the drug sensitivity test showed that while 1 strain was resistant to ampicillin and cefazolin,1 strain was resistant to ampicillin and 3 strains were resistant to cefazolin,the other strains were sensitive to all of the tested drugs,and there was no multi-drug resistance.Conclusion The food poisoning was caused by mixed infection of two different serotypes O2:K28 and O8:K21 of Vibrio parahaemolyticus.

关 键 词：副溶血性弧菌 罕见血清型 混合感染 食物中毒 

分 类 号：R521.1[医药卫生—内科学]