动性球菌产生胞外脂肪酶的条件  

Conditions for Plancoccus Migula Yielding Lipase in Vitro

作　　者：王海晶[1] 梅子青[1] 刘贤忠[1] 黄宜兵[1] 朴真三[1] 

机构地区：[1]吉林大学生命科学学院,长春130023

出　　处：《吉林大学学报（理学版）》2002年第4期430-432,共3页Journal of Jilin University:Science Edition

基　　金：吉林省科委基金(批准号:98.1-09).

摘　　要：测定了动性球菌产生胞外脂肪酶的酶活力,对动性球菌产生胞外脂肪酶的条件进行了研究.结果表明,当碳源为葡萄糖,氮源为蛋白胨,初始pH值为6.0时脂肪酶产率最高;K2HPO4,KH2PO4,CaCl2,ZnSO4和NaCl对脂肪酶产率有促进作用;CuSO4和MgSO4对脂肪酶产率有一定影响;FeCl3,BaCl2,CoSO4和MnSO4对脂肪酶产率影响较大;非离子表面活性剂吐温和聚乙烯醇均能促进脂肪酶的生成,且吐温比聚乙烯醇效果更好.We mensurated the lipase activity of Plancoccus Migula fermentation liquid, and studied the effects of all kinds of growth elements on the lipase yield. The result shows that the optimum conditions to yield lipase are that carbon source is glucose; nitrogen source is peptone; initial pH is 6.0, and while K2HPO4, KH2PO4, CaCl2, ZnSO4 and NaCl can promote lipase production; CuSO4 and MgSO4 can partly decrease lipase output; FeCl3, BaCl2, CoSO4 and MnSO4 inhibit lipase production completely. In addition nonionic surfaceactive agents for instance Tween also promote lipase production.

关 键 词：动性球菌 胞外脂肪酶 酶活力 变压器介质油 产酶条件 产酶率 

分 类 号：TQ925.6[轻工技术与工程—发酵工程] TQ92