Main alkaloids of Rhizoma Coptidis improved palmitic acidinduced insulin resistance in HepG2 cells via AMPK and MAPK signaling pathway  

作　　者：Ming Qu Ying Wang Shijie Cao Yalu Liu Da Liu Feng Qiu Ning Kang 

机构地区：[1]Department of Biochemistry,School of Integrative Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China [2]Tianjin State Key Laboratory of Modern Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China [3]School of Chinese Materia Medica,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China [4]Faculty of Life Sciences and Biological Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China

出　　处：《Asian Journal of Traditional Medicines》2020年第5期239-254,共16页亚洲传统医药（英文）

基　　金：supported by Technology Major Project of China“Key New Drug Creation and Manufacturing Program”(No.2017ZX09301012-001);the National Natural Science Foundation of China(No.20972098,No.81703776 and No.81430095);the National Basic Research Program of China(No.2014CB560706).

摘　　要：Rhizoma Coptidis,a traditional Chinese herbal medicine,has been used for treating diabetes for thousands of years.However,the molecular basis for this action has not been elucidated.In the present study,the effects of seven main alkaloids of Rhizoma Coptidis on glycometabolism were investigated and the related molecular mechanism of five active compounds on insulin resistant(IR)cell model was explored for the first time.Results showed that berberine,palmatine,epiberberine,columbamine and groenlandicine enhanced glucose consumption in the palmitic acid(PA)-induced IR-HepG2 cells,indicating that these compounds could improve IR.In addition,we found that among these active alkaloids,berberine,columbamine,epiberberine and groenlandicine could inhibit the activation of ERK and p38 pathway,while berberine,columbamine,palmatine and epiberberine could activate AMPK pathway.Moreover,palmatine and columbamine regulated the mRNA expression of GLUT2 to ameliorate IR via activating AMPK and inactivating p38 MAPK signal pathway.To sum up,berberine,columbamine,palmatine,epiberberine and groenlandicine might be the active reagents,which contribute to the glucose lowering effects of Rhizoma Coptidis.

关 键 词：Rhizoma Coptidis ALKALOIDS insulin resistance AMPK pathway MAPK pathway GLUT2 

分 类 号：R28[医药卫生—中药学]