携带AFP的rAAV感染树突状细胞对肝癌细胞杀伤作用的实验研究  

The experimental study of DC infected by rAAV with AFP on hepatocellular carcinoma killing effect

作　　者：王斌[1] 付尧 齐红[2] 于鸿[2] 魏天雪 刘玉侠[2] WANG Bin;FU Yao;QI Hong(Jilin Province Tumor Hospital,Changchun130012,China)

机构地区：[1]吉林省肿瘤医院,吉林长春130012 [2]吉林省肿瘤防治研究所

出　　处：《中国实验诊断学》2020年第10期1708-1710,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨携带甲胎蛋白(AFP)的重组腺相关病毒(rAAV)感染树突状细胞(DCs)对肝癌细胞株SMMC-7721的体外杀伤作用,为肝癌非手术治疗提供方法。方法首先确定AFP基因序列并合成AFP基因,将其转染至重组腺相关病毒rAAV,之后将转染AFP的rAAV感染树突状细胞,并将感染rAAV的DC与SMMC-7721共同培养,同时设未感染rAAV的DC作为对照组,用MTT法检测rAAV-DC及DC对SMMC-7721的杀伤活性。结果感染rAAV的DC对SMMC-7721的杀伤活性为89.00%,DC对SMMC-7721的杀伤活性为75.19%,两者比较有差异(P<0.05)。结论携带AFP的rAAV感染DC后,能明显增强DC对SMMC-7721的杀伤活性。Objective To investigate killing effect of dendritic cells(DC)infected by rAAV with AFP on liver cancer cell line-smmc-7721,to provide a new method for treatment of hepatocellular carcinoma.Methods First,the AFP gene was synthesized and transfected into recombinant adeno-assosiated virus(rAAV).Then the rAAV with AFP infected dendritic cells(DCs),and DC infected by rAAV with AFP were co-cultured with SMMC-7721.At the same time,the DC that not infected by rAAV as control group.The killing activity of rAAV-DC and DC on smmc-7721was detected by MTT assay.Results The killing activity of DC infected by rAAV-AFP on smmc-7721was 89.00%.The killing activity of DC not infected by rAAV-AFP on SMMC-7721was 75.19%,there was an obvious difference between the two(P<0.05).Conclusion After rAAV with AFP infected DC,it can significantly enhance the killing activity of DC to SMMC-7721.

关 键 词：甲胎蛋白 重组腺相关病毒 树突状细胞 杀伤活性 

分 类 号：R735.7[医药卫生—肿瘤]