鉴定用于诊断和评估急性早幼粒细胞白血病的挥发性有机化合物  

Identification of Volatile Organic Compounds Used to Diagnose and Evaluate Acute Promyelocyte Leukemia

作　　者：唐红霞 刘志强 戴霁菲 陆燕 夏海龙[1,2] TANG Hong-Xia;LIU Zhi-Qiang;DAI Ji-Fei;LU Yan;XIA Hai-Long(Department of Hematology,Chaohu Hospital of Anhui Medical University,Chaohu 238001,Anhui Province,China;Department of Hematology,First Affiliated Hospital of Anhui Medical University,Hefei 230032,Anhui Province,China;Medical Physics and Technology Center,Hefei Academy of Physical Sciences,Chinese Academy of Sciences,Hefei 230031,Anhui Province,China)

机构地区：[1]安徽医科大学附属巢湖医院血液科,安徽巢湖238001 [2]安徽医科大学第一附属医院血液科,安徽合肥230032 [3]中国科学院合肥物理科学研究院医学物理与技术中心,安徽合肥230031

出　　处：《中国实验血液学杂志》2020年第6期1848-1852,共5页Journal of Experimental Hematology

基　　金：安徽省高校自然科学基金(KJ2017A163)。

摘　　要：目的:研究急性早幼粒细胞白血病(APL)患者呼气成分中的挥发性有机化合物(VOC)特征,探讨VOC用于APL诊断及疗效评估的可行性。方法:以APL患者和健康人为研究对象,采用SPME-GC/MS技术检测受试者呼出气体中的VOC;比较分析APL初诊组、复发组、缓解组和健康组呼气中的VOC成分,使用Kruskal-Wallis方差分析APL不同疾病状态组特征性VOC差异并应用Dunn-Bonferroni检验进行两两比较。结果:初诊APL患者的二甲基硫醚、甲苯和十二烷值明显高于健康人,而乙醇、正己烷和苯甲醛值明显低于健康人(P<0.05)。与初诊组相比,缓解组二甲基硫醚、甲苯和十二烷明显减少,乙醇、正己烷和苯甲醛明显增加(P<0.05),而复发组的VOC与缓解组正好相反。结论:二甲基硫醚、甲苯、十二烷、乙醇、苯甲醛和正己烷可作为APL诊断和病情评估的生物标记物。Objective:To analyze the characteristics of volatile organic compounds(VOCs)in expiratory air components of patients with acute promyelocytic leukemia(APL),and assess the feasibility of VOCs for the diagnosis and prognostic evaluation of APL.Methods:The VOCs exhaled from the patients with APL and healthy volunteers should be analyzed with SPME-GC/MS,and compared between newly-diagnosed group,relapse group,remission group,and healthy group with Wilcoxon/Kruskal-Wallis one-way analysis of variance and Dunn-Bonferroni test.Results:Dimethyl sulfide,toluene,and dodecane obtained of newly-diagnosed APL patients were significantly higher,while ethanol,n-hexanal,and benzaldehyde were significantly lower than those of healthy people(P<0.05).Compared with the newly-diagnosed group,dimethylsulfide,toluene,and dodecane of the remission group significantly decreased,while ethanol,n-hexanal,and benzaldehyde significantly increased(P<0.05),which was just opposite from the relapse group.Conclusion:Dimethyl sulfide,toluene,dodecane,ethanol,n-hexanal,and benzaldehyde can be used as biomarkers for the diagnosis and prognosis assessment of APL patients.

关 键 词：急性早幼粒细胞白血病 生物标志物 挥发性有机化合物 固相微萃取 气相色谱/质谱法 

分 类 号：R733.71[医药卫生—肿瘤]