食用油中苯并(a)芘检测的样品前处理方法——相转移催化皂化法  

Phase transfer catalyzed saponification pretreatment method of sample for the determination of benzo(a)pyrene in edible oil

作　　者：孙长玲 孙嘉彤 胡蒋宁 SUN Changling;SUN Jiatong;HU Jiangning(National Center of Seafood Engineering and Technology,College of Food Science,Dalian Polytechnic University,Dalian 116034,Liaoning,China)

机构地区：[1]大连工业大学食品学院,国家海洋食品工程与技术研究中心,辽宁大连116034

出　　处：《中国油脂》2020年第11期113-115,共3页China Oils and Fats

基　　金：“十三五”国家重点研发计划(2018YFC1602306)。

摘　　要：建立了食用油中苯并(a)芘的相转移催化皂化提取前处理方法,并结合高效液相色谱(HPLC-FLD)进行含量检测。结果表明:该方法检出限为0.2 ng/mL,定量限为0.7 ng/mL,苯并(a)芘平均回收率为96.15%~103.2%,相对标准偏差为2.61%~4.96%。该方法具有降低有机溶剂的消耗、降低检测成本、操作简单、检测时间短等优点,符合快速、便捷、准确及灵敏地检测食用油中苯并(a)芘要求。A phase transfer catalyzed saponification pretreatment method for the determination of benzo(a)pyrene in edible oil by high performance liquid chromatography with FLD(HPLC-FLD) was established. The results showed that the detection limit of the method was 0.2 ng/mL, the quantification limit was 0.7 ng/mL, the average recovery was 96.15%-103.2%, and the relative standard deviation was 2.61%-4.96%.The method had the advantages of reducing the consumption of organic solvents, reducing detection costs, simple operation, short detection time, etc., and met the requirements for rapid, convenient, accurate and sensitive detection of benzo(a)pyrene in edible oil.

关 键 词：食用油 相转移催化皂化 前处理 HPLC-FLD BAP 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.6[轻工技术与工程—食品科学与工程]