基于高灵敏度荧光衍生剂的痕量维生素C液相检测方法建立  

Determination of Trace Vitamin C in Liquid Phase Based on High Sensitivity Fluorescence Derivative

作　　者：沈海波 张连钢 周鑫魁 方剑儒 洪霞 刘善鑫 莫慧娟 张婧蕾 SHEN Hai-bo;ZHANG Lian-gang;ZHOU Xin-kui;FANG Jian-ru;HONG Xia;LIU Shan-xin;MO Hui-juan;ZHANG Jing-lei(Jiayuguan City,Province Food Medicine and Medical Equipment Inspection and Testing Center,Jiayuguan 735100,China;Gansu Institute of Business and Technology,Lanzhou 730010,China;Zhangye City,Pharmaceutical Inspection and Testing Center,Zhangye 734000,China)

机构地区：[1]嘉峪关市食品药品和医疗器械检验检测中心,甘肃嘉峪关735100 [2]甘肃中商食品质量检验检测有限公司,甘肃兰州730010 [3]张掖市药品检验检测中心,甘肃张掖734000

出　　处：《食品工业科技》2020年第23期272-276,283,共6页Science and Technology of Food Industry

摘　　要：利用1.0 g/L 6-羟基-2,4,5-三氨基嘧啶作为荧光衍生剂,与水溶液中维生素C在pH为6.5±0.5反应条件下,40℃衍生反应30 min生成具有强荧光性的衍生产物。试验中对维生素C提取条件,衍生条件,色谱条件进行探索性研究及优化。在使用pH=4.0乙酸铵缓冲液(v)+乙腈(v)+甲醇(v)=80+15+5作为流动相的色谱条件下,该衍生物色谱行为稳定,并产生很强的荧光信号。配合高效液相色谱-荧光检测器对其含量进行检测。该方法检测维生素C具有检测结果准确,回收率为90.9%~96.7%,实验数据稳定RSD≤2%,灵敏度高方法检出限0.2μg/g,检测线性范围0.05~500μg/g,相关性系数良好,y=93322x-1837.8,R^2=0.9999,实验操作简便,无需重复制备空白样品操作等特点,更适合实验室批量检测任务。并对6-羟基-2,4,5-三氨基嘧啶作为荧光衍生剂的使用提供一定的参考。Using 1.0 g/L 6-hydroxy-2,4,5-traminopyrimidine as the fluorescence derivative,the derivatives with strong fluorescence were generated at 40℃for 30 min under the condition of reaction with vitamin C in aqueous solution at a pH of 6.5±0.5.The extraction,derivation and chromatographic conditions of vitamin C were studied and optimized.Under the condition of using pH=4.0 ammonium acetate buffer(v)+acetonitrile(v)+methanol(v)=80+15+5 as the mobile phase,the derivative had stable chromatographic behavior and generates a strong fluorescence signal.Its content was detected by high performance liquid chromatography-fluorescence detector.Vitamin C with accurate result,the method recovery was 90.9%~96.7%,experimental data RSD≤2%or less stable,high sensitivity method detection limit of 0.2μg/g,detection of linear range 0.05~500μg/g,good correlation coefficient,y=93322x-1837.8,R 2=0.9999,experimental operation was simple,did’t need to repeat the preparation of blank sample operation and other characteristics,was more suitable for laboratory batch testing tasks.Some references were provided for the use of 6-hydroxy-2,4,5-traminopyrimidine as a fluorescence derivative.

关 键 词：6-羟基-2 4 5-三氨基嘧啶 荧光衍生 维生素C 高效液相色谱-荧光检测器 

分 类 号：TS207.3[轻工技术与工程—食品科学]