一种三联乳酸菌的体外降胆固醇和抗氧化能力研究  

Study on the Cholesterol-lowering and Antioxidant Abilities of a Tri-lactobacillus In Vitro

作　　者：黄元霞 彭传海 丁宁 邱忠平[1] 李星[1] 邹美慧 HUANG Yuan-xia;PENG Chuan-hai;DING Ning;QIU Zhong-ping;LI Xing;ZOU Mei-hui(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031;China Railway Group,the Second Board of the Central Hospital,Chengdu 610081)

机构地区：[1]西南交通大学生命科学与工程学院,成都610031 [2]中铁二局中心医院,成都610081

出　　处：《生物技术通报》2020年第12期113-120,共8页Biotechnology Bulletin

基　　金：国家自然科学基金面上项目(51978576);四川省重点研发项目(2019YFN0027)。

摘　　要：为获得一种易在体内定植且降胆固醇和抗氧化能力较强三联乳酸菌,以干酪乳杆菌(Lactobacillus casei S1)、屎肠球菌(Enterococcus faecium S4)和哈伯氏乳杆菌(Lactobacillus harbinensis S6)3株供试菌株制备三联乳酸菌,考察三联乳酸菌的人工胃肠耐受性、降胆固醇能力、γ-氨基丁酸(Gamma-aminobutyric acid,GABA)含量和抗氧化活性。结果表明,三联乳酸菌在人工胃肠液处理24 h后,存活率为47.88%,活菌数为1.25×10^9 CFU/mL;总胆固醇去除率、胆盐水解酶(Bile salt hydrolase,BSH)比活以及GABA含量分别为73.43%、151.40 U/mg、1.387 mg/mL;还原力、超氧自由基清除率、羟自由基清除率分别为1.52、95.68%、89.93%,显著高于对照组(P<0.01)。三联乳酸菌的人工胃肠液耐受较强,且具有较强的降胆固醇和抗氧化能力,可进一步开发为功能性微生态制剂或保健品。In order to obtain a tri-lactobacillus that is easy to colonize in vivo and has strong ability of lowering cholesterol and antioxidation,three strains of Lactobacillus casei S1,Enterococcus faecium S4 and Lactobacillus harbinensis S6 were used to form tri-lactobacillus.The artificial gastrointestinal tolerance,cholesterol-lowering ability,yield of γ-aminobutyric acid(GABA),and antioxidant activity of trilactobacillus were investigated.The results showed that the survival rate of tri-lactobacillus was 47.88%and the number of viable bacteria was 1.25×10^9 CFU/mL after 24 h of treatment with artificial gastric intestinal fluid.The total cholesterol removal rate,specific activity of bile salt hydrolase and GABA yield were 73.43%,151.40 U/mg and 1.387 mg/mL,respectively;and the reducing power,superoxide radical clearance rate and hydroxyl radical clearance were 1.52,95.68%and 89.93%,respectively.It was significantly higher than the control group(P<0.01).The tolerance of tri-lactobacillus to artificial gastric and intestinal fluid is strong,and it has strong ability of lowering cholesterol and anti-oxidation,thus it can be further developed as functional microecological preparation or health care product.

关 键 词：三联乳酸菌 人工胃肠液耐受性 降胆固醇 Γ-氨基丁酸 抗氧化活性 

分 类 号：TS201.3[轻工技术与工程—食品科学]