还原氧化石墨烯分子印迹传感器的研制及其在磺胺间甲氧嘧啶检测中的应用  

Fabrication of reduced graphene oxide molecularly imprinted sensor and its application in the detection of sulfamonomethoxine

作　　者：汪洪武 刘宇琪 姚夙 刘艳清 WANG Hong-Wu;LIU Yu-Qi;YAO Su;LIU Yan-Qing(School of Food&Pharmaceutical Engineering,Zhaoqing University,Zhaoqing 526061,China;Guangdong Demonstration Center for Food and Drug Experimental Engineering Education,Zhaoqing University,Zhaoqing 526061,China)

机构地区：[1]肇庆学院食品与制药工程学院,肇庆526061 [2]广东省食品农产品安全分析与检测工程技术研究中心,肇庆526061

出　　处：《食品安全质量检测学报》2020年第22期8463-8469,共7页Journal of Food Safety and Quality

基　　金：广东省教育厅创新团队项目(2017KCXTD032);广东省大学生创新创业训练计划项目(S201910580081、S201910580082)。

摘　　要：目的建立一种印迹电化学传感器快速测定磺胺间甲氧嘧啶的方法。方法以磺胺间甲氧嘧啶为目标分子,在弱碱性条件下,利用多巴胺在还原氧化石墨烯(reduced grapheme oxide,rGO)修饰后的玻碳电极上自聚制得分子印迹聚合膜。采取循环伏安法和差分脉冲伏安法分别对印迹膜的传感性能进行综合研究。结果在最佳的优化条件下,磺胺间甲氧嘧啶在20~1600 nmol/L的浓度范围内与峰电流具有良好线性关系,相关系数r^2=0.9964,检出限为6.7 nmol/L (S/N=3)。实际样品测定的加标回收率为92.4%~108.3%。结论该方法准确、灵敏,适合于实际样品中磺胺间甲氧嘧啶残留的测定。Objective To establish an imprinted electrochemical sensor method for the rapid determination of sulfamonomethoxine. Methods Sulfamonomethoxine was selected as the target molecule. The molecularly imprinted polymer films were prepared by using dopamine self-aggregation on glassy carbon electrodes modified with reduced graphene oxide(rGO) under weakly alkaline conditions. The performance of the molecularly imprinted film sensor was studied by cyclic voltammetry and differential pulsed voltammetry respectively. Results Under the optimal conditions, the peak current was linearly related to the concentration of sulfamonomethoxine in the range of 20-1600 nmol/L, with correlation coefficient r^2=0.9964. The detection limit was 6.7 nmol/L(S/N=3).The standard recovery of the real sample was 92.4%-108.3%. Conclusion The proposed method is accurate and sensitive, which is suitable for the detection of sulfamonomethoxine residues in actual samples.

关 键 词：分子印迹 电化学传感器 还原氧化石墨烯 磺胺间甲氧嘧啶 兽药残留 

分 类 号：O657.1[理学—分析化学] TS207.3[理学—化学]