α桐酸对舌鳞状细胞癌CAL-27细胞的体外抑制作用  

Inhibitory effect ofα-eleostearic acid on tongue squamous cell carcinoma CAL-27

作　　者：王子昱 丁宇洁 陈冰 沈昊翀 孟箭 仝伟凤 WANG Zi-yu;DING Yu-jie;CHEN Bing;SHEN Hao-chong;MENG Jian;TONG Wei-feng(School of Stomatology,Xuzhou Medical University,Xuzhou 221004;School of Medical Imaging,Xuzhou Medical University,Xuzhou 221004;School of Public Health,Xuzhou Medical University,Xuzhou 221004;School of Clinical Medicine,Xuzhou Medical University,Xuzhou 221009,Jiangsu Province,China)

机构地区：[1]徐州医科大学口腔医学院,江苏徐州221004 [2]徐州医科大学医学影像学院,江苏徐州221004 [3]徐州医科大学公共卫生学院,江苏徐州221004 [4]徐州医科大学徐州临床学院口腔科,江苏徐州221009

出　　处：《中国口腔颌面外科杂志》2020年第6期490-494,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：江苏省卫生计生委科研课题(H2017080);徐州市科技局重点研发项目(KC17196);江苏省高等学校大学生创新创业训练计划项目(201810313007Z);基础医学国家级实验教学示范中心(徐州医科大学)资助项目。

摘　　要：目的:探讨苦瓜籽油中的α桐酸(α-eleostearic acid,α-ESA)对舌鳞状细胞癌CAL-27细胞增殖、迁移、凋亡的影响。方法:将0、70、80、90、100、110、120μmol/L的α-ESA培养CAL-27细胞后,用CCK-8法和流式细胞术分别检测加药24、48、72 h后细胞增殖抑制率和加药48 h后细胞凋亡率。再分别以0、80、100μmol/L的α-ESA培养CAL-27细胞不同时间后,用细胞划痕实验检测加药24 h后细胞的迁移能力,Hoechst 33258染色在荧光显微镜下观察加药48 h后凋亡细胞形态。采用SPSS 21.0软件包对实验数据进行统计学分析。结果:α-ESA对CAL-27细胞的增殖抑制作用呈明显时间和浓度依赖性(P<0.05)。作用24、48、72 h后,半数抑制浓度(IC50)逐渐降低,分别为(123.48±1.00)、(80.22±0.03)和(69.27±80.34)μmol/L。流式细胞检测结果显示,细胞凋亡率随着α-ESA浓度的增加而大幅增高(P<0.05)。培养24 h后,加入80、100μmol/L的α-ESA的细胞划痕愈合率分别为(40.08±2.19)%、(22.06±2.04)%,显著低于对照组(74.74±2.17)%(P<0.01)。荧光显微镜下观察到致密浓染或碎块状亮蓝色荧光的细胞凋亡现象。结论:α-ESA能够抑制CAL-27细胞的体外增殖及迁移能力,并可诱导细胞凋亡,从而产生一定的抗肿瘤生长作用。PURPOSE:To investigate the effect ofα-eleostearic acid(α-ESA)in bitter melon seed oil on the proliferation,migration and apoptosis of tongue squamous cell carcinoma CAL-27 cells.METHODS:CAL-27 cells were cultivated in vitro andα-ESA was added at the concentrations of 0,70,80,90,100,110 and 120μmol/L.CCK-8 assay and flow cytometry were used to detect the cell proliferation inhibition rate at 24,48,and 72 h and apoptosis rate at 48 h respectively.CAL-27 cells were treated withα-ESA at concentrations of 0,80 and 100μmol/L.The cell migration capacity after 24 hours ofα-ESA administration was measured by cell scratching assay.The morphology of apoptotic cells after 48 h ofα-ESA administration was observed by Hoechst 33258 staining.SPSS 21.0 software package was used for statistical analysis.RESULTS:The proliferation of CAL-27 cells was inhibited byα-ESA in a time and concentrationdependent manner(P<0.05).After 24,48,and 72 h ofα-ESA administration,the half inhibitory concentration(IC50)was(123.48±1.00)μmol/L,(80.22±0.03)μmol/L,and(69.27±80.34)μmol/L,respectively,which was gradually decreased.The results of flow cytometry showed that the apoptosis rate increased significantly with the increase ofα-ESA concentration(P<0.05).After 24 h of incubation,the wound healing rate of CAL-27 cells was(40.08±2.19)%and(22.06±2.04)%after administration of 80 and 100μmol/Lα-ESA,which was significantly lower than the control group(74.74±2.17)%(P<0.01).Densely stained or fragmented bright blue fluorescence was observed under fluorescence microscope which represented cell apoptosis.CONCLUSIONS:α-ESA can inhibit the proliferation and migration of CAL-27 cells in vitro,and therefore has certain anti-tumor effects.

关 键 词：苦瓜籽油 α桐酸 α-ESA 舌鳞状细胞癌 CAL-27 细胞增殖 迁移 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]