基于响应面法优化酿酒酵母培养体系  

Optimization of Saccharomyces cerevisiae culture system based on response surface method

作　　者：李婷婷 陈雪 程涛[1] 刘斌 孙喆 甄玉国 秦贵信[1] 张学峰 王涛[1,2] LI Tingting;CHEN Xue;CHENG Tao;LIU Bin;SUN Zhe;ZHEN Yuguo;QIN Guixin;ZHANG Xuefeng;WANG Tao(Jilin Provincial Key Laboratory of Animal Production and Product Quality and Safety,Ministry of Education,Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science,College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;JAU-Borui Dairy Science and Technology R&D Center,Jilin Agricultural University,Changchun 130118,China;Feed Engineering Technology Research Center of Jilin Province,Changchun Borui Agriculture and Animal Husbandry Group Co.,Ltd.,Changchun 130118,China;Vocational Technical School of Shuangliao City,Shuangliao 136402,China)

机构地区：[1]吉林农业大学动物科学技术学院,吉林省动物营养与饲料科学重点实验室动物生产及产品质量安全省部共建教育部重点实验室,吉林长春130118 [2]吉林农业大学吉农博瑞奶牛科技研发中心,吉林长春130118 [3]长春博瑞农牧集团股份有限公司吉林省饲料工程技术研究中心,吉林长春130118 [4]双辽市职业中专,吉林双辽136402

出　　处：《中国酿造》2020年第11期126-131,共6页China Brewing

基　　金：吉林省科技发展计划重大科技成果转化项目(20160301003NY)。

摘　　要：该试验通过响应面法对酿酒酵母(Saccharomyces cerevisiae)培养体系进行优化,以达到工业生产上提高酿酒酵母发酵生物量的目的。通过单因素试验确定酿酒酵母培养体系最适碳源为淀粉糖,最适氮源为玉米浆+尿素,最适缓冲盐及微量元素为K2HPO4、MgSO4,最适B族维生素为VB2、VB3、VB8;利用Plackett-Burman试验确定影响酿酒酵母生物量的主因素为淀粉糖、玉米浆、尿素,并应用爬坡试验获得主要因素的峰值范围;最后应用Box-Behnken试验、响应面分析对主要因素进行优化。结果表明,最适宜的酿酒酵母培养体系为:淀粉糖28.8 g/100 mL、玉米浆6.1 g/100 mL、尿素0.0975 g/100 mL、磷酸氢二钾0.1 g/100 mL、硫酸镁0.05 g/100 mL、VB20.1 mg/100 mL、VB30.1 mg/100 mL、VB80.1 mg/100 mL。在优化后培养体系下,酿酒酵母菌液OD560 nm值为60.60,是常规使用的酵母浸出粉胨葡萄糖培养体系的5.27倍。The culture system of Saccharomyces cerevisiae was optimized by response surface methodology,in order to improve the fermentation biomass of S.cerevisiae in industrial production.According to the single-factor experiments,results showed that the optimal condition for S.cerevisiae culture system was starch sugar as carbon source,corn pulp and urea as nitrogen source,K2HPO4 as buffer salt and MgSO4 as trace elements,and the optimal vitamin B group was VB2,VB3 and VB8.The main factors affecting the biomass of S.cerevisiae were starch sugar,corn pulp and urea through the Plackett-Burman design and the peak range of the main factors were obtained by the steep climb path experiments.Box-Behnken design and response surface methodology were used to optimize the main factors.The results showed the optimal fermentation conditions were starch sugar 28.8 g/100 ml,corn pulp 6.1 g/100 ml,urea 0.0975 g/100 ml,K2HPO40.1 g/100 ml,MgSO40.05 g/100 ml,vitamin B20.1 mg/100 ml,vitamin B30.1 mg/100 ml,and vitamin B80.1 mg/100 ml.Under the optimized cultivation system,the OD560 nm value of S.cerevisiae was 60.60,which was 5.27 times as much as that of by yeast extract peptone dextrose medium.

关 键 词：酿酒酵母 培养体系 优化 响应面法 

分 类 号：S816.6[农业科学—饲料科学]