蛇龙珠卷叶病毒病原鉴定和对果实品质的影响  

Pathogen identification of Cabernet Gernischet grapevine leaf-roll virus and its effect on fruit quality

作　　者：刘万好 于素珍 肖慧琳[1] 郑秋玲[1] 宋建强 宋志忠 宫磊 唐美玲[1,2] LIU Wanhao;YU Suzhen;XIAO Huilin;ZHENG Qiuling;SONG Jianqiang;SONG Zhizhong;GONG Lei;TANG Meiling(Yantai Agricultural Science and Technology Institute,Yantai 265500,China;College of Life Science,Yantai University,Yantai 264005,China;School of Agricultural,Ludong University,Yantai 264025,China;Shandong Academy of Grape,Jinan 250100,China)

机构地区：[1]山东省烟台市农业科学研究院,山东烟台265500 [2]烟台大学生命科学学院,山东烟台264005 [3]鲁东大学农学院,山东烟台264025 [4]山东省葡萄研究所,山东济南250100

出　　处：《中国酿造》2020年第11期187-191,共5页China Brewing

基　　金：烟台市葡萄与葡萄酒局专项资金(50012305073);基于物联网的设施葡萄精准调控高效栽培模式建立与示范应用(2018NS10);胶东特优果品创新工程1.0。

摘　　要：以烟台蓬莱的蛇龙珠为试验材料,研究叶片反卷变红对果实品质的影响,同时利用小核糖核酸(sRNA)测序鉴定卷叶病毒病原种类。结果表明,叶片反卷变红症状的蛇龙珠成熟果实中还原糖、总酚和花色苷含量显著减少,含酸量增加。通过sRNA测序鉴定出叶片反卷的蛇龙珠携带有7种已知病毒和4种类病毒,比起常规反转录-聚合酶链反应(RT-PCR),其检测结果更精确,不仅确定了病毒种类,还确定了类病毒种类,以及未知的病毒。Using the Cabernet Gernischet in Yantai Penglai area as the test material,the effect of leaf backscrolling and redness on fruit quality was investigated,and viral pathogens of grapevine leaf-roll disease was identified by small ribonucleic acid sequencing.The results showed that the content of reducing sugar,total phenol and anthocyanin significantly decreased,and the acid content increased in the mature fruit of the Cabernet Gernischet with the symptoms of leaf backscrolling and redness.Through small RNA sequencing,7 known viruses and 4 types of virus-like were identified,compared with conventional reverse transcription-polymerase chain reaction(RT-PCR),the detection results were more accurate,not only determining the type of virus,but also the type of virus-like and unknown viruses.

关 键 词：蛇龙珠 葡萄卷叶病 病毒 果实品质 小核糖核酸测序 

分 类 号：S663.1[农业科学—果树学]