马铃薯资源晚疫病抗性的全基因组关联分析  

Genome-wide association analysis for late blight resistance of potato resources

作　　者：蒋伟 潘哲超[1,2] 包丽仙 周福仙[2] 李燕山 隋启君 李先平[1,2] JIANG Wei;PAN Zhe-Chao;BAO Li-Xian;ZHOU Fu-Xian;LI Yan-Shan;SUI Qi-Jun;LI Xian-Ping(Industrial Crops Research Institute,Yunnan Academy of Agricultural Science,Kunming 650205,Yunnan,China;Scientific Observing and Experimental Station of Potato and Rapeseed in Yunnan-Guizhou Plateau,Kunming 650200,Yunnan,China)

机构地区：[1]云南省农业科学院经济作物研究所,云南昆明650205 [2]云贵高原马铃薯与油菜科学观测实验站,云南昆明650200

出　　处：《作物学报》2021年第2期245-261,共17页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31760409,31460368);云南省农业联合重点项目[2017FG001(-012)];国际马铃薯中心合作项目TON(14.1432.5-001.00)资助。

摘　　要：广谱抗性基因的挖掘是马铃薯高抗晚疫病品种选育的基础。本研究以288份国际马铃薯中心筛选的晚疫病抗性群体为试验材料,经过连续2年田间调查,计算AUDPC和sAUDPC值,评估群体晚疫病抗性;利用SLAF-seq方法进行群体简化基因组测序,通过对晚疫病抗性表型数据的全基因组关联分析,挖掘晚疫病抗性相关的遗传位点和候选基因,为晚疫病抗性品种选育和抗病机理研究提供一定的理论和材料基础。结果表明,晚疫病抗性在288份材料间存在着广泛的遗传差异;基于5种分析模型,共鉴定到82个与晚疫病抗性显著关联的位点;在关联区间关联到54个已知或可能与晚疫病抗性相关的基因。其中,23个基因为抗性基因,包括晚疫病抗性基因R1同源基因、Sw-5同源基因(R8)和Rpi-vnt1以及编码多效性耐药蛋白基因;5个基因编码MAPK蛋白和WRKY转录因子;1个基因参与茉莉酸途径;3个基因与水杨酸途径相关;6个基因是病程相关的基因;3个基因参与苯基丙酸类合成途径;其他与晚疫病抗性相关的基因,如HMGR基因(2个)、细胞色素P450(21个)。The mining of broad-spectrum resistance genes is the basis of the breeding of potato varieties with high late blight resistance.In this study,288 samples of late disease resistance selected by the International Potato Center(CIP)for years were planted and were investigated for two consecutive years,which could provide some theoretical and material basis for breeding of late blight resistant varieties and help to study on disease resistance mechanism.The late blight resistance of each sample was evaluated using AUDPC and sAUDPC values.The population simplified genome sequencing was performed by SLAF-seq and carried out by genome-wide association analysis in order to discover the genetic loci and candidate genes related to late blight resistance.There were wide genetic differences in resistance to late blight among 288 samples.Through genome-wide association analysis,based on five analytical models,a total of 82 loci significantly correlated with late blight resistance were identified.Combined with genomic annotation,there were 54 candidate genes known or likely to be associated with late blight resistance in the association interval.Among them,23 genes were resistant genes,including late blight resistance gene R1 homolog,Sw-5 homolog(R8)and Rpi-vnt1,as well as gene encoding pleiotropic resistance protein.Five genes encoded MAPK protein and WRKY transcription factor;one gene was involved in the JA pathway;three genes were associated with salicylic acid pathway;six genes were related to the course of the disease;three genes were involved in the synthesis of phenylpropionic acid.Other genes related to resistance to late blight included HMGR gene(2)and cytochrome P450(21).

关 键 词：马铃薯 晚疫病抗性 全基因组关联分析 抗性基因 国际马铃薯中心 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]