三叶崖爬藤块根多糖复合酶法提取工艺优化及其抗氧化活性研究  

Polysaccharide Extracted from Root Tuber of Tetrastigma hemsleyanum by Multienzymes and Its Antioxidant Activity

作　　者：胡传久[1] 韩素芳[1] 魏海龙[1] 邹景泉[1] 贺亮[1] 付立忠[2] 程俊文[1] HU Chuan-jiu;HAN Su-fang;WEI Hai-long;ZOU Jing-quan;HE Liang;FU Li-zhong;CHENG Jun-wen(Zhejiang Academy of Forestry,Key Laboratory of Biological and Chemical Utilization of Forest Resources,Hangzhou 310023,China;College of Pharmaceutical Science,Zhejiang Chinese Medical University,Hangzhou 311402,China)

机构地区：[1]浙江省林业科学研究院,浙江省森林资源生物与化学利用重点实验室,浙江杭州310023 [2]浙江中医药大学药学院,浙江杭州311402

出　　处：《浙江林业科技》2020年第6期24-30,共7页Journal of Zhejiang Forestry Science and Technology

基　　金：浙江省科技厅公益项目(2017C32029);浙江省自然科学基金项目(LQ18C160005)。

摘　　要：在单因素试验的基础上,通过Box-Benhnken中心组合设计和响应面分析法优化三叶崖爬藤Tetrastigma hemsleyanum根中多糖的复合酶法提取工艺条件。在前期预实验的基础上,采用淀粉酶、木瓜蛋白酶、果胶酶按2∶2∶1(质量比)组成复合酶对三叶崖爬藤根进行酶解处理,对工艺中的主要参数酶解温度、加酶量和酶解时间进行了优化。响应面分析结果表明,三叶崖爬藤根中多糖的最佳提取条件为:酶解温度51.7℃,加酶量2.8%,酶解时间92.3 min,在此工艺下,验证试验多糖得率为3.84%,与理论值3.97%基本吻合。抗氧化实验表明,三叶崖爬藤多糖对DPPH·,·O2^-,·OH清除作用的IC50分别为0.32,0.46,0.37 mg·mL^-1,表明三叶崖爬藤多糖具有一定的抗氧化能力。In November 2017,root tubers of 3-year Tetrastigma hemsleyanum were collected in Pan’an,Zhejiang province.Experiments were conducted on extraction of polysaccharide from tubers by multienzymatic method and response surface analysis.Based on the results of single factor experiment,enzymolysis was carried out by multienzymes(ratio of amylase,protease,pectinase of 2:2:1).Optimization of extraction technology was implemented on enzymatic hydrolysis temperature,time and total enzyme amount.The response surface analysis demonstrated that polysaccharide yield topped 3.84%under condition of enzymatic hydrolysis temperature of 51.7℃,total enzyme amount of 2.8%,enzymatic hydrolysis time of 92.3 minutes,similar to theoretical value of 3.97%.Antioxidant activity test showed that the polysaccharide from tubers of T.hemsleyanum had the IC50 of radical scavenging capacity on DPPH·,O2^-and OH was 0.32,0.46,0.37 mg/mL.

关 键 词：三叶崖爬藤 多糖 复合酶 响应面 抗氧化 

分 类 号：R284.2[医药卫生—中药学]