非小细胞肺癌患者外周血初始T细胞及记忆T细胞亚群分布  

Distribution of naïve T cell and memory T cell subsets in peripheral blood of non-small cell lung cancer patients

作　　者：刘云鹤[1] 李文涛[1] 于建春[1] Liu Yunhe;Li Wentao;Yu Jianchun(Department of Oncology,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300381,China)

机构地区：[1]天津中医药大学第一附属医院肿瘤科,300381

出　　处：《国际肿瘤学杂志》2020年第9期524-529,共6页Journal of International Oncology

基　　金：天津市教委科研计划(2018KJ034)。

摘　　要：目的分析非小细胞肺癌(NSCLC)患者外周血初始T细胞及记忆T细胞亚群分布情况。方法选取2018年6月至12月天津中医药大学第一附属医院肿瘤科NSCLC患者25例,同期健康志愿者20例作为对照,应用流式细胞仪分析外周血初始T细胞及记忆T细胞亚群含量,所得结果应用SPSS 16.0进行统计分析。结果NSCLC患者初始T细胞/干细胞样中央记忆T细胞(TN/SCM)百分率及绝对数均明显低于对照组[(7.71±1.11)%vs.(15.84±2.00)%,t=3.685,P=0.001;(8.38±1.23)×107/L vs.(3.40±0.43)×108/L,t=6.130,P<0.001],中央记忆T细胞(TCM)百分率及绝对数均明显低于对照组[(6.62±1.16)%vs.(17.88±0.83)%,t=7.641,P<0.001;(7.98±1.78)×107/L vs.(3.40±0.31)×108/L,t=9.028,P<0.001],CD8+TCM细胞绝对数明显低于对照组[(5.19±1.04)×106/L vs.(1.49±0.15)×107/L,t=5.561,P<0.001]。NSCLC患者效应记忆T细胞(TEM)百分率明显高于对照组[(38.27±2.01)%vs.(17.37±1.06)%,t=8.776,P<0.001],CD8+TEM细胞百分率及绝对数均明显高于对照组[(13.93±1.55)%vs.(4.65±0.52)%,t=5.310,P<0.001;(1.48±0.14)×108/L vs.(9.97±1.14)×107/L,t=2.584,P=0.014],终末分化效应记忆T细胞(TEMRA)百分率明显高于对照组[(17.33±1.86)%vs.(8.48±1.01)%,t=3.989,P<0.001]。Ⅰ~Ⅱ期NSCLC患者CD8+TCM细胞百分率及绝对数均明显低于Ⅲ~Ⅳ期患者[(0.33±0.06)%vs.(0.89±0.34)%,t=2.600,P=0.020;(3.99±0.84)×106/L vs.(9.03±3.07)×106/L,t=2.270,P=0.040]。不同病理类型NSCLC患者的初始T细胞及记忆T细胞亚群差异均无统计学意义(均P>0.05)。结论NSCLC患者初始/记忆T细胞亚群较健康志愿者发生变化,具有分化能力和长期存活特性的TN/SCM及TCM降低,而且Ⅰ~Ⅱ期患者降低更为明显,以发挥免疫效应为主的TEM及TEMRA明显升高。Objective To investigate the distribution of naïve T cell and memory T cell subsets in the peripheral blood of non-small cell lung cancer(NSCLC)patients.Methods A total of 25 NSCLC patients from the Oncology Department of the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine from June to December 2018 were included in the study,and 20 healthy volunteers in the same period were used as controls.Flow cytometry was used to analyze the naïve T cell and memory T cell subsets in peripheral blood.The results were analyzed by SPSS 16.0.Results The percentage and absolute number of naïve T cell/stem-like central memory T cells(TN/SCM)in NSCLC patients declined compared to the control group[(7.71±1.11)%vs.(15.84±2.00)%,t=3.685,P=0.001;(8.38±1.23)×107/L vs.(3.40±0.43)×108/L,t=6.130,P<0.001].The percentage and absolute number of central memory T cells(TCM)in NSCLC patients declined compared to the control group[(6.62±1.16)%vs.(17.88±0.83)%,t=7.641,P<0.001;(7.98±1.78)×107/L vs.(3.40±0.31)×108/L,t=9.028,P<0.001].The absolute number of CD8+TCM in NSCLC patients declined compared to the control group[(5.19±1.04)×106/L vs.(1.49±0.15)×107/L,t=5.561,P<0.001].The percentage of effector memory T cells(TEM)in NSCLC patients increased compared to the control group[(38.27±2.01)%vs.(17.37±1.06)%,t=8.776,P<0.001].The percentage and absolute number of CD8+TEM in NSCLC patients increased compared to the control group[(13.93±1.55)%vs.(4.65±0.52)%,t=5.310,P<0.001;(1.48±0.14)×108/L vs.(9.97±1.14)×107/L,t=2.584,P=0.014].The percentage of effector memory T cells re-expressing CD45RA(TEMRA)in NSCLC patients increased compared to the control group[(17.33±1.86)%vs.(8.48±1.01)%,t=3.989,P<0.001].The percentage and absolute number of CD8+TCM in stageⅠ-Ⅱpatients declined compared to stageⅢ-Ⅳpatients[(0.33±0.06)%vs.(0.89±0.34)%,t=2.600,P=0.020;(3.99±0.84)×106/L vs.(9.03±3.07)×106/L,t=2.270,P=0.040].There were no statistically significant differences in the naïve T cells and memory T

关 键 词：癌 非小细胞肺 初始T细胞 记忆T细胞亚群 

分 类 号：R734.2[医药卫生—肿瘤]