利用水苏糖的肠道细菌的分离鉴定及其利用特性研究  

Isolation and identification of the intestinal bacteria capable of utilizing stachyose and its utilization characteristics

作　　者：邬佳颖 毛丙永[1] 谷佳玉 崔树茂[1] 张秋香[1] WU Jiaying;MAO Bingyong;GU Jiayu;CUI Shumao;ZHANG Qiuxiang(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品与发酵工业》2020年第24期16-23,共8页Food and Fermentation Industries

基　　金：国家自然科学基金(31972086);江苏高校品牌专业建设工程项目(PPZY2015A052)。

摘　　要：以水苏糖作为唯一碳源,从健康成人粪便中筛选到12株能利用水苏糖的肠道细菌,包括肺炎克雷伯氏菌、屎肠球菌、粪肠球菌和产气荚膜梭状芽孢杆菌。通过测定细菌培养上清液中水苏糖和棉籽糖含量以及产气情况,结合α-半乳糖苷酶活力测定的结果,发现不同菌株对水苏糖的利用方式存在差异。基因组草图分析发现,菌株对水苏糖的利用方式可能有2种:以产气荚膜梭状芽孢杆菌为代表,利用msmEFG转运体转运水苏糖,再由α-半乳糖苷酶和β-呋喃果糖苷酶水解为单糖再利用;以屎肠球菌、粪肠球菌为代表,利用msmEFG转运体转运水苏糖,由α-半乳糖苷酶将其水解为半乳糖和棉籽糖,并将不利用的棉籽糖释放到细胞外。该实验结果对水苏糖与人体健康关系的研究具有指导意义。Stachyose was used as the only carbon source and 12 strains of intestinal bacteria were isolated from healthy adult feces, which were confirmed to be capable of utilizing stachyose, including Klebsiella pneumoniae , Enterococcus faecalis , Enterococcus faecium and Clostridium perfringen s. These strains can use stachyose in different ways according to the content of stachyose and raffinose in the bacterial culture supernatant, the gas production, and the α-galactosidase activity. Combined with the results of genome analysis, the strains used stachyose in two possible ways. Some bacteria such as Clostridium perfringen s can intake stachyose by msmEFG transporter, which is then hydrolyzed by α-galactosidase and β-fructofuranosidase. Other bacteria such as Enterococcus faecalis and Enterococcus faecium can intake stachyose by msmEFG transporter, hydrolyze stachyose into a galactose, and release the unused raffinose outside of the cell. This study will be valuable for the further research of the relationships between stachyose and human health.

关 键 词：水苏糖 肠道菌 Α-半乳糖苷酶 基因组草图 

分 类 号：TS201.3[轻工技术与工程—食品科学]