DDAH2(-449 G/C) G allele is positively associated with leukoaraiosis in northeastern China: a double-blind, intergroup comparison, case-control study  

作　　者：Ying Fan Qiang Gao Jia-Xin Guan Lei Liu Ming Hong Li Jun Li Wang Hai-Feng Ding Li-Hong Jiang Bo-Yu Hou Mei Li Zhi-Qiang Song De-Qin Sun Chao-Qi Yan Lan Ma 

机构地区：[1]Department of Geriatrics,The 2nd Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang Province,China [2]Department of Geriatrics,Tongling Municipal Hospital,Tongling,Anhui Province,China [3]Department of Cardiovascular Medicine,Shanxi Cardiovascular Hospital,Taiyuan,Shanxi Province,China [4]Physical Examination Center,The 2nd Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang Province,China

出　　处：《Neural Regeneration Research》2021年第8期1592-1597,共6页中国神经再生研究（英文版）

基　　金：supported by the Foundation of Harbin Science Technology Bureau of China,No. 2014RFQGJ042 (to YF);Harbin Medical University Scientific Research Innovation Fund of China,No. 2016LCZX06 (to QG);the Natural Science Foundation of Heilongjiang of China,No. H2016027 (to QG)。

摘　　要：Cerebrovascular endothelial dysfunction is involved in the progression of leukoaraiosis. Asymmetric dimethylarginine is a competitive inhibitor of nitric oxide, which is highly expressed in patients with leukoaraiosis. Dimethylarginine dimethylaminohydrolase(DDAH) is a hydrolytic enzyme that is primarily responsible for eliminating asymmetric dimethylarginine, and it plays a role in the pathogenesis of cardiovascular and cerebrovascular diseases. The DDAH2 subtype is expressed in organs rich in induced nitric oxide synthase, including the heart, the placenta, and the cerebral endothelium during cerebral ischemia, in the stress state, or under neurotoxicity. Overexpression of the DDAH2 gene can inhibit asymmetric dimethylarginine-induced peripheral circulating endothelial cell dysfunction. However, it is unknown whether this polymorphism regulates plasma asymmetric dimethylarginine levels in patients with leukoaraiosis. In this doubleblind study, we recruited 46 patients with leukoaraiosis and 46 healthy, matched controls. Plasma asymmetric dimethylarginine levels were determined using enzyme-linked immunoassays. Genomic DNA was isolated from whole blood samples, and polymerase chain reaction, Sma I restriction enzyme digestion, restriction fragment length polymorphisms, and agarose electrophoresis were used to detect DDAH2(-449 G/C) gene polymorphisms. The results revealed that 95.65% of leukoaraiosis patients had recessive genetic models(GG and CG), while 89.13% of healthy control subjects had dominant genetic models(CC and CG). There was a significant difference in the genotype composition ratio between leukoaraiosis patients and healthy controls(P = 0.0002). The frequency of G alleles in the leukoaraiosis patients(71.74%) was significantly higher than in healthy controls, whereas the frequency of C alleles was lower(χ~2 = 13.9580, P = 0.0002). Furthermore, asymmetric dimethylarginine concentrations in subjects with the GG genotype were significantly higher than in subjects with the CG and CC genotypes(Kruskal

关 键 词：LEUKOARAIOSIS dimethylarginine dimethylaminohydrolase 2 gene polymorphism ALLELE asymmetric dimethylarginine nitric oxide endothelial dysfunction cerebrovascular diseases clinical trial 

分 类 号：R743[医药卫生—神经病学与精神病学]