显色法测定甘孜雪菊总黄酮含量  

Determination of Total Flavonoids in Ganzi Snow Chrysanthemum by Chromogenic Method

作　　者：胡继红[1] 王静霞[1] 李俊儒[1] HU Ji-hong;WANG Jing-xia;LI Jun-ru(Sichuan Technology&Business College,Dujiangyan Sichuan 611830,China)

机构地区：[1]四川工商职业技术学院,四川都江堰611830

出　　处：《食品与发酵科技》2020年第6期131-135,共5页Food and Fermentation Science & Technology

摘　　要：芦丁为标准品,弱碱条件下铝盐为显色剂,用紫外分光光度法对黄酮类物质含量进行测定,在2-20μg/mL浓度范围内,线性良好,回收率89.3-99.6%,RSD:0.8-4.7%。分别用90%乙醇和沸水对甘孜雪菊和杭白菊进行提取,用该法对不同品种菊花中总黄酮含量进行测定。结果表明:甘孜雪菊中测出的总黄酮(31.5%)含量远高于杭白菊(6.84%),也比文献报道的新疆雪菊的含量至少高1.5倍。沸水浸泡得到甘孜雪菊与杭白菊浸出率分别是11.53%和1.65%,说明用泡茶方式可有效摄入菊花中的黄酮类物质,但摄入量仅为总量的1/3。利用紫外分光光度计扫描不同菊花提取液,甘孜雪菊与杭白菊都含有黄酮类物质,但黄酮种类不完全相同。Rutin was used as the standard,aluminum salt as the chromogenic agent under the condition of weak base.The content of flavonoids was determined by ultraviolet spectrophotometry.The linearity was good in the concentration range of 2-20μg/mL,the recovery was 89.3-99.6%,RSD was 0.8-4.7%.90%percent ethanol and boiling water were used to extract and determine the content of total flavonoids in different chrysanthemum species.The results showed that the content of total flavonoids(31.5%)in Ganzi snow chrysanthemum was much higher than that in hang bai chrysanthemum(6.84%)and at least 1.5 times higher than that in Xinjiang snow chrysanthemum.The extraction rates of Garze and hangbai chrysanthemum were 11.53%and 1.65%respectively,which indicated that the flavonoids in Chrysanthemum could be effectively absorbed by tea making,but the intake was only one third of the total amount.Using UV spectrophotometer to scan different chrysanthemum extracts,there are flavonoids in both Garze snow chrysanthemum and hangbai chrysanthemum,but the flavonoids are not the same.

关 键 词：雪菊 总黄酮 比较 

分 类 号：TS207.3[轻工技术与工程—食品科学]