超高效液相色谱-串联质谱法测定畜肉中阿托品  

Determination of Atropine in Meat by Ultra-high Performance Liquid ChromatographyTandem Mass Spectrometry

作　　者：杜国辉[1] 姜冬峰 张应龙[1] DU Guo-hui;JIANG Dong-feng;ZHANG Ying-long(Shandong Institute of Commerce and Technology,Jinan 250103,China)

机构地区：[1]山东商业职业技术学院,山东济南250103

出　　处：《食品与药品》2020年第6期495-497,共3页Food and Drug

摘　　要：目的建立畜肉中阿托品含量超高效液相色谱-串联质谱(UPLC-MS/MS)检测方法。方法畜肉中阿托品经乙腈水提取,PRiMEHLB净化,采用Waters ACQUITYUPLC®BEH C18色谱柱分离,在正离子模式下进行电喷雾离子源电离,采用多反应监测模式扫描。结果阿托品在0.5〜50μg/kg范围内线性关系良好。在0.5,1.0,5.0μg/kg 3个添加水平的回收率范围为82.6%〜102.8%。阿托品定量限为0.5μg/kg。结论该方法简单、灵敏、准确,可作为畜肉中阿托品的检测方法之一。Objective To establish a method for the determination of atropine in meat by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods Atropine in meat was treated with acetonitrilewater extraction,then purified by PRiME HLB.The samples were separated with Waters ACQUITY UPLC®BEH C18 column(2.1 mm×100 mm,1.7μm).The analysis was performed by MS/MS with electrospray ionization in positive ion mode with multiple reaction monitoring(MRM).Results Atropine showed a good linearity at the range of 0.5~50μg/kg.The recoveries ranged from 82.6%to 102.8%at the spikes of 0.5,1.5 and 5.0μg/kg.The quantification limit of atropine was 0.5μg/kg.Conclusion This method has the advantages of simple pretreatment,sensitivity and accuracy,and it can be regarded as the technique of determination of atropine in meat.

关 键 词：超高效液相色谱-串联质谱 阿托品 畜肉 

分 类 号：R155.5[医药卫生—营养与食品卫生学]