青叶苎麻叶多酚超声辅助提取工艺优化及抗氧化活性研究  

Research on the optimization of ultrasonic assisted extraction technology and antioxidant activities of polyphenols extracted from green leaves ramie

作　　者：张晓婷 王满生[2,3] 邱浩楠 叶凤凌 职士淇 陈龙 何强[1] 王延周[1] 董怡 ZHANG Xiao-ting;WANG Man-sheng;QIU Hao-nan;YE Feng-ling;ZHI Shi-qi;CHEN Long;HE Qiang;WANG Yan-zhou;DONG Yi(College of Biomass Science and Engineering,Sichuan University,Chengdu,Sichuan 610065,China;Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,Hunan 410205,China;Key Laboratory of Biological and Processing for Bast Fiber Crops,MOA,Changsha,Hunan 410205,China)

机构地区：[1]四川大学轻工科学与工程学院,四川成都610065 [2]中国农业科学院麻类研究所,湖南长沙410205 [3]农业部麻类生物学与加工重点实验室,湖南长沙410205

出　　处：《食品与机械》2020年第12期152-158,共7页Food and Machinery

基　　金：高校基本科研项目(编号:20826041D4060);湖南省自然科学基金(编号:2020JJ5640);中央级公益性科研院所基本科研业务费专项(编号:1610242020004)。

摘　　要：优化了青叶苎麻叶多酚的提取工艺参数,并以没食子酸为对照评价了青叶苎麻叶多酚的抗氧化活性。结果表明:优化出的多酚提取工艺参数分别为料液比(m苎麻∶V乙醇)1∶33(g/mL)、乙醇体积分数40%、提取温度42℃,在此条件下青叶苎麻多酚提取量为47.97 mg/g。青叶苎麻叶多酚清除DPPH自由基和ABTS+自由基的IC50值分别为(0.15±0.00),(2.28±0.03)μg/mL,氧自由基吸收能力为(42.41±0.01)g Trolox/g多酚,均明显优于没食子酸,具有良好的抗氧化效果。In this study,the optimum extraction parameters of polyphenols from leaves of Boehmeria nivea var.tenacissima were optimized,and the antioxidant activity of polyphenols from ramie leaves was evaluated by using gallic acid as a control.Results of polyphenols extraction experiment showed that the ultrasonic power and ultrasonic time have less effect on the amount of polyphenols extraction,and the influence of three factors on polyphenols extraction was ordered as:ethanol concentration(significantly)>extraction temperature>solid-liquid ratio.Finally,the optimized polyphenols extraction process parameters were verified and determined as:1∶33(g/mL)of liquid-solid ratio,40%of ethanol concentration and 42℃of the extraction temperature and the amount of polyphenols extraction could reach at 47.97 mg/g under this conditions.The results of antioxidant activity evaluation showed that polyphenols from leaves of green leaf ramie had good antioxidant effects,and the DPPH free radical scavenging ability[IC50 value(0.15±0.00)g/mL],ABTS free radical scavenging ability[IC50 value(2.28±0.03)g/mL]and oxygen free radical absorbing ability[ORAC value(42.41±0.01)g Trolox equivalent/g polyphenol]were significantly superior to gallic acid.

关 键 词：青叶苎麻 多酚 超声辅助提取 抗氧化 

分 类 号：R284.2[医药卫生—中药学]