芦子提取物对两种致龋菌的抑制作用  

Inhibitory Effect of the Extracts of Piper betle's Fruit on Two Kinds of Cariogenic Bacteria

作　　者：徐磊 王丽梅 顾绍魁 陈雨薇 蓝海 Xu Lei;Wang Limei;Gu Shaokui;Chen Yuwei;Lan Hai(College of Pharmacy,Dali University,Dali,Yunnan 671000,China;Department of Stomatology,Dali Bai Autoromous Prefecture People′s Hospital,Dali,Yunnan 671000,China;Lincang People’s Hospital,Lincang,Yunnan 677000,China;Public Basic Teaching Department,West Yunnan University of Applied Sciences,Dali,Yunnan 671006,China)

机构地区：[1]大理大学药学院,云南大理671000 [2]大理白族自治州人民医院口腔科,云南大理671000 [3]临沧市人民医院,云南临沧677000 [4]滇西应用技术大学公共基础教学部,云南大理671006

出　　处：《大理大学学报》2020年第12期91-93,共3页Journal of Dali University

基　　金：国家自然科学基金项目(81260512)。

摘　　要：目的:研究芦子乙酸乙酯萃取部位对两种致龋菌(变形链球菌、黏性放线菌)的生长和代谢产酸的影响。方法:通过二倍稀释法,以洗必泰为阳性对照组,测定芦子乙酸乙酯萃取部位对两种致龋菌的最低抑菌浓度(MIC)、最低杀菌浓度(MBC);测定芦子乙酸乙酯萃取部位低于MIC 4个浓度对两种致龋菌代谢产酸的影响。结果:芦子乙酸乙酯萃取部位对变形链球菌和黏性放线菌的MIC值分别为6.250、3.125 mg/mL,MBC值分别为12.500、6.250 mg/mL;芦子乙酸乙酯萃取部位4个浓度对黏性放线菌代谢产酸均有抑制作用,高浓度组对变形链球菌的产酸有抑制作用。结论:芦子乙酸乙酯萃取部位对黏性放线菌的抑制作用强于变形链球菌;抑制产酸的作用和浓度成正相关,但效果均弱于阳性对照组。Objective:The effects of extraction site of ethyl acetate of Piper betle's fruit on the growth and metabolism of two kinds of cariogenic bacteria(Streptococcus mutans and Actinomyces viscosus)were studied.Methods:The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of two kinds of cariogenic bacteria in the extraction site of ethyl acetate were determined by double dilution method with Chlorhexidine as the positive control group.The effects of 4 concentrations of ethyl acetate extraction lower than MIC on the metabolism of two kinds of cariogenic bacteria were determined.Results:The MIC values of the extraction site of ethyl acetate against Streptococcus mutans and Actinomyces viscosus were 6.250 mg/mL and 3.125 mg/mL respectively,and the MBC values were 12.500 mg/mL and 6.250 mg/mL respectively.All the 4 concentrations at the extraction site of ethyl acetate inhibited the acid production of Actinomyces viscosus,and the high concentration group inhibited the acid production of Streptococcus mutans.Conclusion:The inhibition effect of ethyl acetate extraction on Actinomyces viscosus was stronger than that on Streptococcus mutans.The inhibition effect of acid production was positively correlated with the concentrations,but the effects were all weaker than those in the positive control group.

关 键 词：芦子 乙酸乙酯萃取部位 致龋菌 抑菌 产酸 

分 类 号：R781.1[医药卫生—口腔医学]