基于生物信息学数据库分析肾透明细胞癌SKA1表达及临床意义  

Analysis of SKA1 gene expression in clear cell renal cell carcinoma and its clinical significance based on bioinformatics database

作　　者：韩静[1] 袁帅[2] 蒲艳 柳惠斌[1] Han Jing;Yuan Shuai;Pu Yan;Liu Huibin(Institute of Cancer Research,Affiliated Tumor Hospital of Xinjiang Medical University,Urumqi 830011,China;Department of Urology,Affiliated Tumor Hospital of Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学附属肿瘤医院肿瘤防治研究所,乌鲁木齐830011 [2]新疆医科大学附属肿瘤医院泌尿外科,乌鲁木齐830011

出　　处：《国际肿瘤学杂志》2020年第10期598-605,共8页Journal of International Oncology

基　　金：国家自然科学基金(81760453)。

摘　　要：目的探讨纺锤体和动粒相关蛋白复合体亚基1(SKA1)在肾透明细胞癌中的表达及临床意义。方法收集2018年1月至2018年12月在新疆医科大学附属肿瘤医院就诊的76例肾透明细胞癌患者术前和24例健康体检者的静脉血液标本,利用实时荧光定量PCR检测患者全血中SKA1的水平,分析SKA1的水平与临床病理特征的关系。挖掘Oncomine(v4.5)、The Human Protein Atlas(THPA)基因数据库、The Cancer Genome Atlas(TCGA)门户网站(cBioportal)和Gene Expression Omnibus(GEO)中关于SKA1的信息,基于cBioportal数据库中的肾透明细胞癌数据采用Kaplan-Meier法进行生存分析,log-rank检验进行生存率的比较,χ^2检验分析SKA1水平与临床病理特征的关系。采用ROC曲线评价SKA1 mRNA在肾透明细胞癌中的诊断价值,并采用KOBAS 3.0版在线工具对SKA1基因进行富集分析。结果Oncomine(v4.5)数据库中检索到2项关于SKA1 mRNA在人肾透明细胞癌组织中表达水平的研究,共38个样本,分析结果显示人SKA1 mRNA在肾透明细胞癌组织中呈高表达,进一步检测发现,与正常肾组织相比,SKA1 mRNA在肾透明细胞癌组织中的表达水平显著升高[-2.21(-3.56,-1.59)vs.-3.41(-4.55,-1.65)],差异具有统计学意义(Z=2.282,P=0.022)。利用THPA在线网站分析表明,SKA1蛋白在肾癌组织中呈明显中等强度染色,而在正常肾组织中极少部分弱阳性表达或不表达,SKA1主要定位于细胞质膜,这一检测结果与mRNA分析结果一致。cBioportal数据库分析结果表明,SKA1表达水平与AJCC分期(χ^2=21.352,P<0.001)、T分期(χ^2=19.967,P<0.001)、N分期(χ^2=11.323,P=0.003)及M分期(χ^2=27.248,P<0.001)显著相关。76例肾透明细胞癌患者外周血SKA1的水平为0.301±0.147,健康体检者为0.162±0.052,差异具有统计学意义(t=7.360,P<0.001),其水平与AJCC分期(t=2.445,P=0.017)和淋巴结转移(t=2.242,P=0.028)相关,该结果与cBioportal数据库中组织分析结果相符。生存分析显示,cBioportObjective To investigate the expression of spindle and kinetochore-associated complex subunit 1(SKA1)gene in clear cell renal cell carcinoma(ccRCC)and its clinical significance.Methods The venous blood samples of 76 preoperative patients with ccRCC and 24 healthy subjects were collected from the Affiliated Tumor Hospital of Xinjiang Medical University from January 2018 to December 2018.The level of SKA1 in whole blood was detected by real-time fluorescence quantitative PCR,and the relationship between SKA1 level and clinicopathological characteristics was analyzed.SKA1 data were retrieved from Oncomine(v4.5),The Human Protein Atlas(THPA)gene databases,The Cancer Genome Atlas(TCGA)databasec(cBioportal)and Gene Expression Omnibus(GEO).The Kaplan-Meier method was used to perform patients'survival analysis based on cBioportal ccRCC data,and the survival rates were compared by log-rank method.The relationship between SKA1 expression level and clinicopathological characteristics was analyzed by χ^2 test.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of SKA1 mRNA in ccRCC,and enrichment analysis of SKA1 gene was carried out using KOBAS 3.0 online tool.Results Two studies on the expression level of SKA1 mRNA in ccRCC were retrieved from Oncomine(v4.5)database,and there were 38 samples.The results showed that SKA1 mRNA was highly expressed in ccRCC tissues.Further detection showed that the expression level of SKA1 mRNA in ccRCC tissues was significantly higher than that in normal renal tissues[-2.21(-3.56,-1.59)vs.-3.41(-4.55,-1.65)],and there was a statistically significant difference(Z=2.282,P=0.022).The analysis of THPA online website showed that SKA1 protein showed obvious moderate staining in ccRCC tissues,while weakly positive or no expression in normal renal tissues.SKA1 was mainly located in the plasma membrane,which was consistent with the results of mRNA analysis.The results of cBioportal showed that the expression level of SKA1 was significantly correlated with AJCC stagi

关 键 词：肾肿瘤 预后 计算生物学 SKA1 

分 类 号：R737.11[医药卫生—肿瘤] Q811.4[医药卫生—临床医学]