不同木质素对酶蛋白的吸附影响及酶活变化研究  

Adsorption Effects of Lignin on Different Enzyme Proteins

作　　者：牟洪燕[1,2] 刘怡蓓 黄瑾 MOU Hongyan;LIU Yibei;HUANG Jin(State Key Lab of Pulp and Paper Engineering,South China University of Technology,Guangzhou,Guangdong Province,510640;National Experimental Teaching Demonstration Center for Light Industry and Food,South China University of Technology,Guangzhou,Guangdong Province,510640)

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640 [2]华南理工大学轻工与食品国家级实验教学示范中心,广东广州510640

出　　处：《中国造纸学报》2020年第4期23-27,共5页Transactions of China Pulp and Paper

基　　金：国家自然科学基金(31600469);广东省自然科学基金(2017A030332343);制浆造纸工程国家重点实验室2020年度开放基金(202010)。

摘　　要：通过3,5-二硝基水杨酸(DNS)和对硝基苯基-β-D-吡喃葡萄糖苷(pNPG)比色法,研究对比碱法和水溶预处理桉木得到的两种不同木质素对不同酶蛋白(纤维素酶、聚木糖酶和β-葡萄糖苷酶)的吸附和脱附影响差异,以及木质素吸附对不同酶蛋白活性的抑制影响。两种木质素对不同酶蛋白吸附实验表明,温度对碱木质素吸附酶蛋白能力的影响大于二甲苯磺酸钠(SXS)木质素,被吸附的酶蛋白更容易从SXS木质素上脱附下来;木质素吸附会显著降低酶活,升高温度可以提高木质素对酶蛋白的吸附能力,但会抑制酶活。Two kinds of lignin obtained from alkali and water soluble pretreatment of eucalyptus were used as raw materials,and through DNS and pNPG colorimetry,the effects of two kinds of lignin on the adsorption and desorption of different enzyme proteins(cellulase,xylanase,andβ-glucosidase)and the inhibition of lignin adsorption on the activities of different enzyme proteins were studied.The results showed that the effect of temperature on the adsorption capacity of alkali lignin was greater than that of odium xylene sulfonate(SXS)lignin.The experiments of different enzyme proteins adsorbed on two kinds of lignin showed that the adsorbed enzyme proteins on SXS lignin were more easily to be desorbed.After lignin adsorption,the enzyme activity was significantly reduced,and the higher temperature could not only enhance the adsorption capacity of the lignins to enzyme proteins,but also inhibit the activity of enzyme.

关 键 词：木质素 纤维素酶 聚木糖酶 Β-葡萄糖苷酶 吸附作用 

分 类 号：TS721[轻工技术与工程—制浆造纸工程] O636.2[理学—高分子化学]