石榴皮总黄酮超临界萃取及萃余物黄色素提取  

Synchronous Extraction of Total Flavonoid from Pomegranate Peel and Yellow Pigment from Supercritical Fluid Extraction Residue

作　　者：尹春光[1] 宋文路[1] 李明丽 刘亚文 王晓强[1] 王森 YIN Chun-guang;SONG Wen-lu;LI Ming-li;LIU Ya-wen;WANG Xiao-qiang;WANG Sen(Department of Biological Science and Engineering,Jining University,Qufu 273155,Shandong,China;College of Food Science,South China Agricultural University,Guangzhou 510640,Guangdong,China)

机构地区：[1]济宁学院生命科学与工程系,山东曲阜273155 [2]华南农业大学食品学院,广东广州510640

出　　处：《食品研究与开发》2021年第1期80-87,共8页Food Research and Development

基　　金：国家重点研发计划项目(2017YFE0122800);山东省重点研发计划项目(2019GSF107061);济宁市重点研发计划项目(2018ZDGH024)。

摘　　要：利用超临界CO2方法萃取石榴皮中总黄酮、三萜类,然后超声波辅助提取萃余物中黄色素。采用正交试验探究萃取时间、压力、温度、夹带剂浓度对总黄酮、三萜类及对超临界残渣黄色素提取率的影响,得到最优工艺条件。超临界萃取总黄酮最优条件为萃取时间70 min,压力30 MPa,温度85℃,夹带剂浓度90%,总黄酮提取率为12.482%,此时萃余物黄色素提取率为5.870%,同时检测到使用超声波辅助提取萃余物黄色素,其提取率极显著高于微波辅助法(P<0.01)。黄色素抗氧化活性检测DPPH自由基清除能力及ABTS+自由基清除能力分别为(27335.16±8.5501)μmol FERA/g和(4164.10±4.6576)μmol Trolox/g。In order to extract the total flavonoids and triterpenoids from pomegranate peel by supercritical CO2,to extract the yellow pigment from the residue by ultrasonic wave,effects of time,entrainer concentration,temperature and pressure on total flavonoids,triterpenoids from pomegranate peel and yellow pigment from residue were investigated by orthogonal experiments.Thus,the optimal extraction conditions of total flavonoids were extraction time 70 min,pressure 30 MPa,temperature 85℃and entrainer concentration 90%,the extraction rate was 12.482%,yellow pigment extraction rate was 5.870%.At the same time,the ultrasonic extraction rate of yellow pigment was significantly higher than that of microwave-assisted extraction(P<0.01).The free radical scavenging capacity of DPPH and ABTS+were detected,the antioxidant activity was(27335.16±8.5501)μmol FERA/g and(4164.10±4.6576)μmol Trolox/g respectively.

关 键 词：总黄酮 三萜类 黄色素 超临界萃取 萃余物 石榴皮 

分 类 号：TS209[轻工技术与工程—食品科学]