Dual-Wavelength Photosensitive Nano-in-Micro Scaffold Regulates Innate and Adaptive Immune Responses for Osteogenesis  

作　　者：Qin Zhao Miusi Shi Chengcheng Yin Zifan Zhao Jinglun Zhang Jinyang Wang Kailun Shen Lingling Zhang Hua Tang Yin Xiao Yufeng Zhang 

机构地区：[1]The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)and Key Laboratory of Oral Biomedicine,Ministry of Education,School and Hospital of Stomatology,Wuhan University,Wuhan 430079,People’s Republic of China [2]Medical Research Institute,School of Medicine,Wuhan University,Wuhan 430071,People’s Republic of China [3]Institute of Immunology,Shandong First Medical University&Shandong Academy of Medical Sciences,Taian 271000,People’s Republic of China [4]Institute of Health and Biomedical Innovation&Australia-China Centre for Tissue Engineering and Regenerative Medicine,Queensland University of Technology,Kelvin Grove 4059,QLD,Australia

出　　处：《Nano-Micro Letters》2021年第2期124-143,共20页纳微快报（英文版）

基　　金：supported by the National Key R&D Program of China(2018YFC1105300);the National Natural Science Foundation of China(81771050,81700931,81901017);the Special Fund for Technical Innovation of Hubei Province(2017AHB046);the Natural Science Foundation of Hubei Province(2017CFA025).

摘　　要：The immune response of a biomaterial determines its osteoinductive effect.Although the mechanisms by which some immune cells promote regeneration have been revealed,the biomaterial-induced immune response is a dynamic process involving multiple cells.Currently,it is challenging to accurately regulate the innate and adaptive immune responses to promote osteoinduction in biomaterials.Herein,we investigated the roles of macrophages and dendritic cells(DCs)during the osteoinduction of biphasic calcium phosphate(BCP)scaffolds.We found that osteoinductive BCP directed M2 macrophage polarization and inhibited DC maturation,resulting in low T cell response and efficient osteogenesis.Accordingly,a dual-targeting nano-in-micro scaffold(BCP loaded with gold nanocage,BCP-GNC)was designed to regulate the immune responses of macrophages and DCs.Through a dual-wavelength photosensitive switch,BCP-GNC releases interleukin-4 in the early stage of osteoinduction to target M2 macrophages and then releases dexamethasone in the later stage to target immature DCs,creating a desirable inflammatory environment for osteogenesis.This study demonstrates that biomaterials developed to have specific regulatory capacities for immune cells can be used to control the early inflammatory responses of implanted materials and induce osteogenesis.

关 键 词：Gold nanocage Drug release Photocatalysis IMMUNOMODULATION OSTEOINDUCTION 

分 类 号：TB383.1[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]